| Autor: |
Jakub Pšenčík, Roman Dědic, František Vácha, Jan Hála, Martin Vacha, M Lovčinský |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
ResearcherID |
| ISSN: |
0022-2860 |
| DOI: |
10.1016/s0022-2860(98)00824-2 |
| Popis: |
Well defined photosystem II reaction centers from Pisum sativum containing 5 or 6 chlorophyll a (Chl a), 2 pheophytine a (Pheo a), and 1 or 2 β -carotene ( β Car) molecules were prepared by using immobilized metal affinity chromatography. Samples containing 6 Chl a and 1 or 2 β -Car and containing 5 Chl a and 1 β -Car were measured using low temperature absorption, fluorescence and hole-burning spectroscopy. Absorption bands of the β Car (462, 490, and 508 nm) can be clearly distinguished next to the Soret absorption band of Chl a at low temperature. Their relative intensities strongly depend on Chl/Car ratio. The shapes of fluorescence bands are the same for all samples. Persistent spectral holes were burnt into both absorption and fluorescence spectra. This technique provides lifetime of excited state τ 1 and Huang–Rhys factor S . Values of τ 1 correspond to two picoseconds energy transfer in reaction centers. Huang–Rhys factor S =0.4 appears to be the same for all studied samples. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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