Status quo of tet regulation in bacteria
| Autor: | Bernd Neumann, Christopher F. Schuster, Ralph Bertram |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Transposable element
Regulation of gene expression Genetics Bacteria Base Sequence Effector Tetracycline Resistance Repressor Bioengineering biochemical phenomena metabolism and nutrition Biology Tetracycline Applied Microbiology and Biotechnology Biochemistry Bacterial genetics DNA binding site chemistry.chemical_compound chemistry Bacterial Proteins Animals TetR Gene Biotechnology Transcription Factors |
| Zdroj: | Microbial biotechnology. 15(4) |
| ISSN: | 1751-7915 |
| Popis: | The tetracycline repressor (TetR) belongs to the most popular, versatile and efficient transcriptional regulators used in bacterial genetics. In the tetracycline (Tc) resistance determinant tet(B) of transposon Tn10, tetR regulates the expression of a divergently oriented tetA gene that encodes a Tc antiporter. These components of Tn10 and of other natural or synthetic origins have been used for tetracycline-dependent gene regulation (tet regulation) in at least 40 bacterial genera. Tet regulation serves several purposes such as conditional complementation, depletion of essential genes, modulation of artificial genetic networks, protein overexpression or the control of gene expression within cell culture or animal infection models. Adaptations of the promoters employed have increased tet regulation efficiency and have made this system accessible to taxonomically distant bacteria. Variations of TetR, different effector molecules and mutated DNA binding sites have enabled new modes of gene expression control. This article provides a current overview of tet regulation in bacteria. |
| Databáze: | OpenAIRE |
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