After insufficient radiofrequency ablation, tumor-associated endothelial cells exhibit enhanced angiogenesis and promote invasiveness of residual hepatocellular carcinoma

Autor: Jun Gao, Wenbing Sun, Lemin Zheng, Shan Ke, Jian Kong, Ling-Qun Kong, Hui-Chuan Sun, Jinge Kong, Xuemei Ding
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Pathology
Neoplasm
Residual
Angiogenesis
Radiofrequency ablation
Hepatocellular carcinoma
lcsh:Medicine
Cell Communication
Cell Separation
Metastasis
law.invention
Neovascularization
law
Medicine
Extracellular Signal-Regulated MAP Kinases
Tube formation
Medicine(all)
Neovascularization
Pathologic
Liver Neoplasms
NF-kappa B
General Medicine
Invasiveness
Up-Regulation
surgical procedures
operative
Catheter Ablation
medicine.symptom
therapeutics
Signal Transduction
STAT3 Transcription Factor
medicine.medical_specialty
Carcinoma
Hepatocellular
Green Fluorescent Proteins
General Biochemistry
Genetics and Molecular Biology
Cell Line
Tumor
Carcinoma
Cell Adhesion
Humans
Neoplasm Invasiveness
Tumor-associated endothelial cells
Protein kinase B
Cell Shape
Cell Proliferation
business.industry
Biochemistry
Genetics and Molecular Biology(all)
Research
lcsh:R
Endothelial Cells
Reproducibility of Results
Hyperthermia
Induced
medicine.disease
business
Proto-Oncogene Proteins c-akt
Zdroj: Journal of Translational Medicine, Vol 10, Iss 1, p 230 (2012)
Journal of Translational Medicine
ISSN: 1479-5876
Popis: Background The mechanism regarding rapid progression of residual hepatocellular carcinoma (HCC) after insufficient radiofrequency ablation (RFA) has been preliminarily discussed. However, most studies have mainly focused on RFA-induced changes in the tumor cells. The present study was designed to determine whether tumor-associated endothelial cells (TAECs) could contribute to the invasiveness of HCC after insufficient RFA. Methods TAECs were isolated from fresh HCC tissue and characterized. Morphological changes were observed in TAECs after heat treatment for 10 min. TAEC proliferation, migration and tube formation after heat treatment for 10 min at 37°C (control group), and 42 and 47°C (insufficient RFA groups) were examined. The differences in TAECs interactions with HepG2-GFP or HCCLM3-GFP cells among the two insufficient RFA groups and control group were evaluated. The expression of E-selectin, ICAM-1 and VCAM-1 in TAECs was measured. The effects of TAECs on the invasiveness of HepG2-GFP or HCCLM3-GFP cells after insufficient RFA were analyzed. The IL-6, IL-8, MCP-1 and GRO-α concentrations in conditioned medium from TAECs were measured after insufficient RFA. The associated signaling pathways of Akt, ERK1/2, STAT3 and NF-κB were analyzed in TAECs after insufficient RFA. Results TAECs expressed the EC-specific markers and took up complexes of Dil-Ac-LDL. Relative to the control group, the proliferation of TAECs was significantly inhibited and their migration and tube formation were significantly enhanced in the insufficient RFA groups. Significantly more HepG2-GFP or HCCLM3-GFP cells adhered to TACEs in these groups than in the control group (all PP Conclusions Insufficient RFA enhanced TAEC migration and tube formation, and this may play a key role in the rapid growth of residual HCC. Increased expression of metastasis-related molecules in TAECs after insufficient RFA may be a potential mechanism for the metastasis of residual HCC.
Databáze: OpenAIRE
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