Subacute stress and chronic stress interact to decrease intestinal barrier function in rats
| Autor: | Shadea Salim Rasoel, Fernando Fornari, Adriana Lauffer, Tim Vanuytsel, Jan Tack, Hanne Vanheel, Ricard Farré, Christophe Vanormelingen, Joran Toth |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Physiology Colon Ileum Biology Occludin Permeability Tight Junctions Jejunum 03 medical and health sciences Behavioral Neuroscience Intestinal mucosa Internal medicine medicine Animals Chronic stress Mast Cells Intestinal Mucosa Rats Wistar Barrier function Intestinal permeability Endocrine and Autonomic Systems medicine.disease Small intestine Rats Psychiatry and Mental health 030104 developmental biology Neuropsychology and Physiological Psychology medicine.anatomical_structure Endocrinology Corticosterone Stress Psychological |
| Zdroj: | Stress (Amsterdam, Netherlands). 19(2) |
| ISSN: | 1607-8888 |
| Popis: | Psychological stress increases intestinal permeability, potentially leading to low-grade inflammation and symptoms in functional gastrointestinal disorders. We assessed the effect of subacute, chronic and combined stress on intestinal barrier function and mast cell density. Male Wistar rats were allocated to four experimental groups (n = 8/group): 1/sham; 2/subacute stress (isolation and limited movement for 24 h); 3/chronic crowding stress for 14 days and 4/combined subacute and chronic stress. Jejunum and colon were collected to measure: transepithelial electrical resistance (TEER; a measure of epithelial barrier function); gene expression of tight junction molecules; mast cell density. Plasma corticosterone concentration was increased in all three stress conditions versus sham, with highest concentrations in the combined stress condition. TEER in the jejunum was decreased in all stress conditions, but was significantly lower in the combined stress condition than in the other groups. TEER in the jejunum correlated negatively with corticosterone concentration. Increased expression of claudin 1, 5 and 8, occludin and zonula occludens 1 mRNAs was detected after subacute stress in the jejunum. In contrast, colonic TEER was decreased only after combined stress, and the expression of tight junction molecules was unaltered. Increased mast cell density was observed in the chronic and combined stress condition in the colon only. In conclusion, our data show that chronic stress sensitizes the gastrointestinal tract to the effects of subacute stress on intestinal barrier function; different underlying cellular and molecular alterations are indicated in the small intestine versus the colon. |
| Databáze: | OpenAIRE |
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