Mucin expression by transitional cell carcinomas of the bladder
| Autor: | M. L. T. H. Samaratunga, B. G. Hohn, Michael A. McGuckin, Michael Walsh, Peter L. Devine, W. Thong, Robert A. Gardiner |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.drug_class Urology Urinary Bladder urologic and male genital diseases Monoclonal antibody digestive system Epithelium Immunoenzyme Techniques medicine Humans Urothelium MUC1 Aged Neoplasm Staging Aged 80 and over Carcinoma Transitional Cell Mucin-2 Urinary bladder Membrane Glycoproteins business.industry Mucin Mucin-1 Mucins Antibodies Monoclonal Middle Aged medicine.disease digestive system diseases Neoplasm Proteins Transitional cell carcinoma medicine.anatomical_structure Urinary Bladder Neoplasms Transitional Cell Cancer research Immunohistochemistry Female business |
| Zdroj: | British journal of urology. 73(3) |
| ISSN: | 0007-1331 |
| Popis: | To examine the presence of a membrane-associated and secreted mucin (MUC1) and a secreted gel-forming mucin (MUC2) in normal and malignant urothelium.Sections were obtained from archival paraffin blocks from 11 patients with nonmalignant urological conditions and 89 patients with transitional cell carcinomas (TCC). Mucin expression was examined by immunohistochemistry using monoclonal antibodies BC2 and 4F1, reactive with epitopes on the protein core of MUC1 and MUC2 respectively.In normal urothelium MUC1 was limited predominantly to the apical membranes of the umbrella cell layer. MUC1 was present in all cases of TCC, and the pattern of expression divided into three categories: luminal membrane staining only, luminal plus cytoplasmic staining of intermediate +/- basal layers, or staining of only isolated cells or cell groups. These staining patterns were significantly associated with both tumour grade and stage (P0.001), with cytoplasmic staining more prevalent in higher grade and stage tumours. MUC2 was not detected in normal urothelium, and was present in 40% of cases of TCC, characterized by intense granular cytoplasmic staining. No association between MUC2 expression and either tumour grade or stage was demonstrated.MUC1 mucin was expressed by both normal and malignant urothelium, with increased expression characteristic of higher grade and stage tumours. MUC2 expression was found in 40% of tumours but not in normal urothelium. The role of these mucins in the biology of the bladder requires further investigation. |
| Databáze: | OpenAIRE |
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