Effects of intracanal carbon dioxide laser irradiation on cultured human fibroblasts

Autor: Hiroshi Nakamura, Akira Senda, Masataka Yamaguchi, Ayako Itoh, Toru Matsumoto, Naoki Horiba
Rok vydání: 2005
Předmět:
Zdroj: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 100:767-771
ISSN: 1079-2104
DOI: 10.1016/j.tripleo.2005.01.012
Popis: The purpose of this study was to evaluate, under various conditions, the damage that might occur to viable cells after CO2 laser irradiation of the root canal.A laser tip was placed within the root canal of extracted human teeth that were positioned above cultured fibroblasts (NB cells), and then irradiation was applied. The irradiation modes used were either pulse (40 pps) or super-pulse (151 pps). The laser energy was set at 32 J in both modes, and the tip of the laser beam was positioned to a point 2 mm from the fibroblasts.When the pulse mode irradiation was applied, there was significant difference (P.05) in the percentage of viable cells between teeth with closed apical foramen (1-mm wall between root canal and root apex) and the #15 group (No. 15 K-file used), #30 group (No. 30 K-file), or #50 group (No. 50 K-file). The difference between the #30 group and the #50 group (P.05) was also significant. The larger the diameter of the apical foramen became, the lower was the percentage of viable cells.If a CO2 laser irradiates a root canal system enlarged to within 1 mm of the root canal length, little damage to the periapical tissues would be expected to occur.
Databáze: OpenAIRE