Lipophilic amino alcohols reduces carrageenan-induced paw edema and anti-OVA DTH in BALB/c mice
| Autor: | Caio César de Souza Alves, Erick Esteves de Oliveira, Mauro V. de Almeida, Tais A. Correa, Elaine F. C. Reis, Ana Paula Ferreira, Sandra B.R. Castro |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male medicine.drug_class Ovalbumin Immunology Interleukin-1beta Anti-Inflammatory Agents Inflammation Pharmacology Carrageenan Nitric Oxide Anti-inflammatory BALB/c Nitric oxide Cell Line chemistry.chemical_compound Mice Edema medicine Immunology and Allergy Animals Hypersensitivity Delayed Dexamethasone Cells Cultured Amino alcohol Mice Inbred BALB C biology Chemistry Foot Tumor Necrosis Factor-alpha Macrophages Allergens biology.organism_classification Amino Alcohols In vitro Paw edema IL-1β TNF-α Anesthesia medicine.symptom medicine.drug |
| Zdroj: | International immunopharmacology. 17(3) |
| ISSN: | 1878-1705 |
| Popis: | The inflammation process is a coordinated response of the organism related to immune response with release of pro-inflammatory substances, as nitric oxide, TNF-α and IL-1β. In this work, a series of lipophilic amino alcohols were evaluated on RAW264.7 and primary macrophages for the modulation of nitric oxide and TNF-α. The most potent compounds were submitted to the treatment of BALB/c mice and evaluation of the carrageenan-induced paw edema and TNF-α and IL1-β release in the paws and anti-OVA delayed type hypersensitivity reaction. RAW264.7 and primary macrophages were incubated in the presence of amino alcohols at different concentrations (1, 0.5, 0.05 and 0.005μgmL−1). All tested compounds were not cytotoxic, however the inhibition of NO and TNF-α were observed only in RAW264.7 cultures. The NO production were reduced in 100% for all compounds, but only the compounds 4a and 4b expressively reduced the TNF-α release (67% and 92% respectively). On the carrageenan-induced paw edema, the compound 4b treatment showed reduction of edema, TNF-α and IL-1β as efficient as dexamethasone treatment. Meanwhile, the compound 4a treatment showed only slight reduction of paw edema. In the anti-OVA DTH reaction, both compounds showed reduction in the paw edema as effective as dexamethasone. In function of the observed results in vitro and in the acute and anti-OVA inflammation of mice paw edema compound 4b showed promissory anti-inflammatory properties. |
| Databáze: | OpenAIRE |
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