Medulloblastoma/primitive neuroectodermal tumour studied as a Matrigel enhanced subcutaneous xenograft model
| Autor: | Vivienne Tobias, Katy Sterling-Levis, Ursula R. Kees, Les White |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Vincristine
Pathology medicine.medical_specialty medicine.medical_treatment Aziridines Mice Nude Antineoplastic Agents Biocompatible Materials Carboplatin Immunophenotyping Mice chemistry.chemical_compound Nude mouse Physiology (medical) Benzoquinones Animals Neuroectodermal Tumors Primitive Medicine Cerebellar Neoplasms Neuroectodermal tumor Antineoplastic Agents Alkylating Cyclophosphamide Medulloblastoma Mice Inbred BALB C Matrigel Diaziquone Chemotherapy biology business.industry General Medicine medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays Drug Combinations Treatment Outcome Neurology chemistry Proteoglycans Surgery Collagen Laminin Neurology (clinical) Cisplatin business medicine.drug |
| Zdroj: | Journal of Clinical Neuroscience. 8:151-156 |
| ISSN: | 0967-5868 |
| Popis: | An important role for pre-clinical models of medulloblastoma/primitive neuroectodermal tumour (MB/PNET) is inhibited by the limitations of conventional heterotransplantation. Nine cohorts of MB/PNET were studied for subcutaneous engraftment in nude mice by both conventional and Matrigel supplemented methods. While no subcutaneous tumours resulted from 63 conventional attempts, an aggregate 41 xenografts from 72 injections (57%) were produced when Matrigel was added to the cell suspension. In subsequent passage, engraftment rate approached 100%. To study the response to chemotherapeutic agents in the model, a total of 221 tumours in 3 cohorts were treated using one of the following: cisplatin, carboplatin, vincristine, cyclophosphamide, diaziquone, or saline control. While all agents demonstrated statistically significant activity, cyclophosphamide proved to be particularly effective. The potential applications of this xenograft model in the biologic as well as therapeutic study of MB/PNET deserve continuing investigation. |
| Databáze: | OpenAIRE |
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