Seven genes that are differentially transcribed in colorectal tumor cell lines
| Autor: | Sebastian Hentsch, Ulrich Finke, Oliver Müller, Ingrid Hoffmann, Mary Pat Moyer, Ute Melchers, Silke Erdmann, Inko Nimmrich |
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| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
Transcription Genetic Molecular Sequence Data Cell Cycle Proteins Receptors Cell Surface Complementary DNA Gene expression Tumor Cells Cultured Guanine Nucleotide Exchange Factors Humans Amino Acid Sequence RNA Neoplasm Northern blot Gene Genetics Differential display Membrane Glycoproteins Sequence Homology Amino Acid biology Reverse Transcriptase Polymerase Chain Reaction Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Proteins Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic Reverse transcription polymerase chain reaction Caldesmon Oncology biology.protein Calmodulin-Binding Proteins Reticulocalbin 1 Cholecystokinin Colorectal Neoplasms |
| Zdroj: | Cancer Letters. 160:37-43 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/s0304-3835(00)00553-x |
| Popis: | To identify genes which are differentially transcribed in colorectal tumor cells, we compared the two human tumor cell lines, SW480 and HCT116, with the cell line, NCM460, from normal colon epithelium as a control. Using the methods of differential display reverse transcription PCR and Northern blot hybridization, we detected the differential transcription of seven genes: cholecystokinin, reticulocalbin, Rab5 guanine nucleotide exchange factor Rabex5, caldesmon, differentiation related gene 1 (drg1), taxol resistant associated gene 3 (Trag-3) and the gene for the placental protein, diff33. The yet unidentified cDNA of the human Rabex5 gene and the 3′ untranslated region of the human caldesmon gene were cloned. |
| Databáze: | OpenAIRE |
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