Screening of highly efficient photosynthetic hybrids of Oryza officinalis and analysis of their photosynthetic pathway genes
| Autor: | S.Q. Xiao, F.Y. Yin, X. Ke, Z.Q. Cheng, C. Li, J.H. You, E.X. Li, D.Y. Kan |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Oxygenase Physiology Introgression Plant Science Biology Photosynthesis 01 natural sciences pcr amplification gene homology Gene Hybrid RuBisCO Botany photosynthetic parameters food and beverages 04 agricultural and veterinary sciences excellent traits Pyruvate carboxylase Biochemistry primer design QK1-989 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries Phosphoenolpyruvate carboxylase 010606 plant biology & botany |
| Zdroj: | Photosynthetica, Vol 59, Iss 2, Pp 303-312 (2021) |
| ISSN: | 1573-9058 0300-3604 |
| Popis: | Hereditary properties of strong growth and huge accumulation of biomass in Oryza officinalis exhibit a great potential; however, the genes that code for its high photosynthesis performance are not established. This study screened eight hybrids, using biomass accumulation and photosynthesis analysis, based on the introgression lines constructed by analyzing distant hybridization patterns between Oryza officinalis and cultivars HY-8. We designed 23 primer pairs using transcriptome sequencing of Oryza officinalis and screened two types of photosynthetic enzymes: phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK), which are two related proteins of ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco), its binding protein (rubis-subs-bind), and a small subunit (rbcS). Results showed that C4 photosynthetic pathway enzymes, PEPC and PPDK, were highly expressed in hybrids and the source plant, Oryza officinalis. Homology analysis also indicated that the sequences of those two genes were different from those of the C3 and C4 plants investigated. Therefore, a better understanding of the photosynthetic characteristics of Oryza officinalis would provide clues for further isolation of valuable genes from this plant. |
| Databáze: | OpenAIRE |
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