High prevalence of asymptomatic Leishmania spp. infection among liver transplant recipients and donors from an endemic area of Brazil
| Autor: | J. R. G. Cortes, J. B. Iodith, L. C. Faria, Roberta Maia de Castro Romanelli, Luciana Inácia Gomes, T. A. M. da Silva, Ana Rabello, Wanessa Trindade Clemente, H. R. Fernandes, S. S. S. Lima, R. V. P. Nunes, Agnaldo Soares Lima, Vanessa Peruhype-Magalhães, K. Z. Protil |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent medicine.medical_treatment Fluorescent Antibody Technique Spleen Liver transplantation Asymptomatic Polymerase Chain Reaction law.invention law parasitic diseases medicine Prevalence Immunology and Allergy Humans Pharmacology (medical) Prospective Studies Leishmania infantum Amastigote Child Direct fluorescent antibody Polymerase chain reaction Aged Leishmania Transplantation biology business.industry DNA Protozoan Middle Aged medicine.disease biology.organism_classification Tissue Donors Liver Transplantation Visceral leishmaniasis medicine.anatomical_structure Cross-Sectional Studies Child Preschool Immunology Leishmaniasis Visceral Female medicine.symptom business Brazil |
| Zdroj: | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 14(1) |
| ISSN: | 1600-6143 |
| Popis: | Visceral leishmaniasis is an uncommon disease in transplant recipients; however, if left untreated, the mortality can be high. If an organ donor or recipient is known to be an asymptomatic Leishmania spp. carrier,monitoring is advised. This study proposes to assess the prevalence of asymptomatic Leishmania spp.infection in liver transplant donors and recipients from an endemic area. A total of 50 liver recipients and 17 liver donors were evaluated by direct parasite search, indirect fluorescent antibody test (IFAT), anti-Leishmania rK39 rapid test and Leishmania spp.DNA detection by polymerase chain reaction (PCR).Leishmania spp. amastigotes were not observed in liver or spleen tissues. Of the 67 serum samples, IFAT was reactive in 1.5% and indeterminate for 17.9%, and the anti-Leishmania rK39 rapid test was negative for all samples. The PCR test was positive for 7.5%, 8.9%, and 5.9% of blood, liver and spleen samples, respectively(accounting for 23.5% of the donors and 8% of the recipients). Leishmania infantum-specific PCR confirmed all positive samples. In conclusion, a high prevalence of asymptomatic L. infantum was observed in donors and recipients from an endemic area, and PCR was the most sensitive method for screening these individuals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text