Use of the Ogawa-Kudoh method to isolate mycobacteria in a tuberculosis reference laboratory in northwestern Paraná, Brazil
| Autor: | Rosangela Zampieri Pina, Vera Lúcia Dias Siqueira, Rosilene Fressatti Cardoso, Aline Lemes Castilho, Elisa Keiko Hirayama Takao, Katiany Rizzieri Caleffi-Ferracioli, Fernanda Schaefer Borges da Silva, Flaviane Granero Maltempe |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Veterinary medicine
Tuberculosis Mycobacterium tuberculosis^i2^sdetec Método de Ogawa-Kudoh^i2^savalia lcsh:RS1-441 Reference laboratory lcsh:Pharmacy and materia medica Age and gender Mycobacterium tuberculosis fluids and secretions medicine Retrospective analysis Tuberculose General Pharmacology Toxicology and Pharmaceutics Mycobacterium tuberculosis^i1^sdetect biology business.industry Gold standard (test) bacterial infections and mycoses medicine.disease biology.organism_classification Ogawa-Kudoh method Ogawa-Kudoh method^i1^sevaluat Immunology Ziehl–Neelsen stain Christian ministry Tuberculosis^i1^sdiagno business Tuberculose^i2^sdiagnóst Método de Ogawa-Kudoh |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Volume: 49, Issue: 3, Pages: 567-570, Published: SEP 2013 Brazilian Journal of Pharmaceutical Sciences, Vol 49, Iss 3, Pp 567-570 (2013) Brazilian Journal of Pharmaceutical Sciences; Vol. 49 No. 3 (2013); 567-570 Brazilian Journal of Pharmaceutical Sciences; Vol. 49 Núm. 3 (2013); 567-570 Brazilian Journal of Pharmaceutical Sciences; v. 49 n. 3 (2013); 567-570 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1984-8250 2175-9790 |
| DOI: | 10.1590/s1984-82502013000300018 |
| Popis: | A cultura é o método padrão ouro para confirmação da tuberculose (TB). O Ministério da Saúde Brasileiro propôs, recentemente, a utilização do método de Ogawa-Kudoh para cultura de escarro na detecção de Mycobacterium tuberculosis. O objetivo deste estudo foi avaliar oito anos de utilização do método de Ogawa-Kudoh na rotina de um laboratório de referência na região noroeste do Paraná, Brasil. Realizou-se estudo retrospectivo dos registros das culturas de escarro para a detecção de micobactérias, usando o método Ogawa-Kudoh conduzido no Laboratório de Bacteriologia Médica, Laboratório de ensino e pesquisa em Análises Clínicas (LEPAC) da Universidade Estadual de Maringá (UEM), de Julho de 2003 a Setembro de 2011. As seguintes variáveis foram analisadas: esfregaço Ziehl Neelsen (Z-N), cultura, idade e sexo do paciente. Analisaram-se 3.231 amostras de escarro de pacientes com suspeita de tuberculose. Destes, 67,17% eram do sexo masculino com idade média de 45,58 anos. Do total de amostras Z-N negativas (n=2.949), 42 amostras (42/2949, 1,42%) apresentaram cultura positiva para M. tuberculosis (p>;0,05). A utilização do método Ogawa-Kudoh representa excelente ferramenta para o diagnóstico precoce da TB pulmonar. É de fácil execução, requer menos equipamentos de biossegurança do que o método de Petroff, apresenta baixo custo e boa sensibilidade para detecção de M. tuberculosis. Culturing is the gold standard method for confirming a diagnosis of tuberculosis (TB). The Brazilian Ministry of Health recently proposed the use of the Ogawa-Kudoh method for sputa cultures to detect Mycobacterium tuberculosis. The aim of the present study was to evaluate 8 years of using the Ogawa-Kudoh method in a TB reference laboratory in northwestern Paraná, Brazil. The present study consisted of a retrospective analysis of sputa cultures records for the detection of mycobacteria using the Ogawa-Kudoh method in the Laboratory of Medical Bacteriology, Laboratory of Teaching and Research in Clinical Analysis (LEPAC), State University of Maringá, from July 2003 to September 2011. The following variables were analyzed: Ziehl Neelsen (Z-N) smears and cultures results and the age and gender of the patients. Sputa samples from 3,231 patients with suspected TB were analyzed. Of these, 67.17% were male with an average age of 45.58 years. Of the total number of Z-N-negative samples (n=2,949), 42 (1.42%) were positive for M. tuberculosis (p >;0.05). The Ogawa-Kudoh method is an excellent tool for diagnosing pulmonary TB. It is easy to perform, requires less biosafety equipment than the Petroff method, has a low cost, and has good sensitivity for detecting of M. tuberculosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|