Gonadotropin-releasing hormone stimulates the biosynthesis of pregnenolone sulfate and dehydroepiandrosterone sulfate in the hypothalamus
| Autor: | Ai Fen Wang, Hubert Vaudry, Georges Pelletier, Jae Young Seong, Van Luu-The, Hyuk Bang Kwon, Yves Tillet, Delphine Burel, Catherine Taragnat, Jean Luc Do-Rego, Jian Hua Li |
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| Přispěvatelé: | Seong, Jae Young, Vaudry, Hubert, ProdInra, Archive Ouverte, Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hormone Research Center, Chonnam National University, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Endocrinologie Moléculaire et Oncologique et Génomique Humaine, Université Laval [Québec] (ULaval), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratory of G-Protein-Coupled Receptors, Korea University [Seoul], The Brain Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2011-0019205) to J.Y.S., a grant from INSERM (U413) to H.V., France-Korea (STAR) exchange program (to J.Y.S. and H.V.), France-Québec (FRSQ-INSERM) exchange program (toG.P.andH.V.), Ministère de l’Enseignement Supérieure de la Recherche et la Région Haute-Normandie, Université Laval, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Korea University, Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pituitary gland Sulfotransferase Ranidae Gonadotropin-releasing hormone Gonadotropin-Releasing Hormone chemistry.chemical_compound 0302 clinical medicine Endocrinology Protein Isoforms hypothalamus In Situ Hybridization Neurons pregnenolone 0303 health sciences Microscopy Confocal Dehydroepiandrosterone Sulfate Reverse Transcriptase Polymerase Chain Reaction dhea Preoptic area medicine.anatomical_structure Hypothalamus Pituitary Gland Pregnenolone Sulfotransferases Pregnenolone sulfate hormones hormone substitutes and hormone antagonists medicine.drug Autre (Sciences du Vivant) hormone grh medicine.medical_specialty [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] endocrine system Molecular Sequence Data Biology Cell Line 03 medical and health sciences Dehydroepiandrosterone sulfate Internal medicine medicine Animals rt pcr Amino Acid Sequence Diencephalon immunofluorescence 030304 developmental biology Sequence Homology Amino Acid [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT] Gene Expression Profiling Sequence Analysis DNA chemistry hybridation in situ 030217 neurology & neurosurgery Receptors LHRH |
| Zdroj: | Endocrinology 6 (154), 2114-2128. (2013) Endocrinology Endocrinology, 2013, 154 (6), pp.2114-2128. ⟨10.1210/en.2013-1095⟩ Endocrinology, Endocrine Society, 2013, 154 (6), pp.2114-2128. ⟨10.1210/en.2013-1095⟩ |
| ISSN: | 0013-7227 |
| DOI: | 10.1210/en.2013-1095⟩ |
| Popis: | L’article original est publié par The Endocrine Society; The sulfated neurosteroids pregnenolone sulfate (Δ(5)PS) and dehydroepiandrosterone sulfate (DHEAS) are known to play a role in the control of reproductive behavior. In the frog Pelophylax ridibundus, the enzyme hydroxysteroid sulfotransferase (HST), responsible for the biosynthesis of Δ(5)PS and DHEAS, is expressed in the magnocellular nucleus (Mgd) and the anterior preoptic area (Poa), two hypothalamic regions that are richly innervated by GnRH1-containing fibers. This observation suggests that GnRH1 may regulate the formation of sulfated neurosteroids to control sexual activity. Double-labeling of frog brain slices with HST and GnRH1 antibodies revealed that GnRH1-immunoreactive fibers are located in close vicinity of HST-positive neurons. The cDNAs encoding three GnRH receptors (designated riGnRHR-1, -2, and -3) were cloned from the frog brain. Reverse transcription-polymerase chain reaction analyses revealed that riGnRHR-1 is strongly expressed in the hypothalamus and the pituitary while riGnRHR-2 and -3 are primarily expressed in the brain. In situ hybridization histochemistry indicated that GnRHR-1 and GnRHR-3 mRNAs are particularly abundant in Poa and Mgd whereas the concentration of GnRHR-2 mRNA in these two nuclei is much lower. Pulse-chase experiments using tritiated Δ(5)P and DHEA as steroid precursors, and 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as a sulfonate moiety donor, showed that GnRH1 stimulates in a dose-dependent manner the biosynthesis of Δ(5)PS and DHEAS in frog diencephalic explants. Since Δ(5)PS and DHEAS, like GnRH, stimulate sexual activity, our data strongly suggest that some of the behavioral effects of GnRH could be mediated via the modulation of sulfated neurosteroid production. |
| Databáze: | OpenAIRE |
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