Microgravity effects on frozen human sperm samples
| Autor: | Antoni Perez-Poch, S Garcia, Daniel Gonzalez, Pere N. Barri, S. Garcia-Monclús, Montserrat Boada, Marta Ballester, Anna Veiga |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament de Ciències de la Computació, Universitat Politècnica de Catalunya. LAM - Laboratori d'Aplicacions Multimèdia i TIC |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cryoprotectant DNA fragmentation Apoptosis DNA Fragmentation Vitality Normal spermatozoa Andrology 03 medical and health sciences Informàtica::Aplicacions de la informàtica [Àrees temàtiques de la UPC] Cryoprotective Agents 0302 clinical medicine Gamete Biology Freezing Genetics Humans Fragmentation (cell biology) Genetics (clinical) Sperm motility Cryopreservation 030219 obstetrics & reproductive medicine Weightlessness Chemistry Obstetrics and Gynecology Motility General Medicine Sperm bank Spermatozoa Sperm Semen Analysis Ambients de microgravetat 030104 developmental biology Reproductive Medicine Sperm Motility Reduced gravity environments Microgravity Semen Preservation Developmental Biology |
| Zdroj: | J Assist Reprod Genet UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) |
| ISSN: | 1573-7330 1058-0468 |
| DOI: | 10.1007/s10815-020-01877-5 |
| Popis: | Purpose: Microgravity has severe effects on cellular and molecular structures as well as on metabolic interactions. The aim of this study is to investigate the effects of microgravity (µg) exposure on human frozen sperm samples. Methods: Sibling samples from 15 normozoospermic healthy donors were frozen using glycerol as cryoprotectant and analyzed under microgravity and ground conditions. Microgravity was obtained by parabolic flights using a CAP10B plane. The plane executed 20 parabolic maneuvers with a mean of 8.5 seconds of microgravity for each parabola. Results: Frozen sperm samples preserved in cryostraws and stored in a secure and specific nitrogen vapor cryoshipper do not suffer significant alterations after µg exposure. Comparing the study group (µg) and the control group (1g), similar results were obtained in the main parameters studied: Sperm motility (M/ml) 13.72 ± 12.57 vs 13.03±12.13 (-0.69 95% CI [-2.9;1.52]); Progressive a+b sperm motility (%) 13 21.83±11.69 vs 22.54±12.83 (0.03 95% CI [-0.08;0.15]); Sperm vitality (%) 46.42±10.81 vs 44.62±9.34 14 (-0.04 95% CI [-0.13;0.05]); Morphologically normal spermatozoa (%) 7.03±2.61 vs 8.09±3.61 (0.12 15 95% CI [0.01;0.24]); DNA sperm fragmentation by SCD (%) 13.33±5.12 vs 13.88±6.14 (0.03 95% CI [- 16 0.09;0.16]); Apoptotic spermatozoa by MACS (%) 15.47±15.04 vs 23.80±23.63 (-0.21 95% CI [- 17 0.66;1.05]). Conclusion: The lack of differences obtained between frozen samples exposed to µg and those maintained in ground conditions provides the possibility of considering the safe transport of human male gametes to space. Nevertheless, further research is needed to validate the results and to consider the possibility of creating a human sperm bank outside the Earth. |
| Databáze: | OpenAIRE |
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