Field amplified sample stacking of amyloid beta (1-42) oligomers using capillary electrophoresis
| Autor: | Sadia Paracha, Christa N. Hestekin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fluid Flow and Transfer Processes Chromatography Resolution (mass spectrometry) biology Amyloid beta 010401 analytical chemistry Biomedical Engineering Stacking Condensed Matter Physics 01 natural sciences 0104 chemical sciences Congo red 03 medical and health sciences Electrophoresis chemistry.chemical_compound 030104 developmental biology Colloid and Surface Chemistry Capillary electrophoresis SPECIAL TOPIC: SELECTED PAPERS FROM THE 2015 ANNUAL MEETING OF THE AES ELECTROPHORESIS SOCIETY IN SALT LAKE CITY UTAH (GUEST EDITORS NATHAN SWAMI AND MICHAEL PYCRAFT HUGHES) chemistry biology.protein General Materials Science Orange G Macromolecule |
| Zdroj: | Biomicrofluidics. 10(3) |
| ISSN: | 1932-1058 |
| Popis: | Oligomeric forms of the amyloid beta (Aβ) protein have been indicated to be an important factor in the development of Alzheimer's disease (AD). Since the oligomeric forms of Aβ can vary in size and conformation, it is vital to understand the early stages of Aβ aggregation in order to improve the care and treatment of patients with AD. This is the first study to determine the effect of field amplified sample stacking (FASS) on the separation of oligomeric forms of Aβ1-42 using capillary electrophoresis (CE) with ultraviolet (UV) detection. UV-CE was able to separate two different species of Aβ1-42 oligomers (75% average reduction) and increased peak heights (>60% average increase) when compared to non-FASS conditions. UV-CE with FASS also provided higher resolution between the Aβ1-42 oligomers for all aggregation time points studied. In addition, Congo red and Orange G inhibition studies were used to help evaluate the conformation of the observed species. This work demonstrates the ability of UV-CE employing FASS to provide higher resolution between oligomeric forms of Aβ1-42 without significantly altering their aggregation. |
| Databáze: | OpenAIRE |
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