The fungal mitochondrial membrane protein, BbOhmm, antagonistically controls hypoxia tolerance
| Autor: | Yongjun Zhang, Xin Zhao, Nemat O. Keyhani, Zhangjiang He, Huifang Wang, Juan Deng, Zhibing Luo, Yifei Gao, Yanze Kan, Zhuoyue Lu |
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| Rok vydání: | 2019 |
| Předmět: |
Insecta
Virulence Biology medicine.disease_cause Microbiology Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Acetyltransferases Gene Expression Regulation Fungal medicine Animals Beauveria Hypoxia Gene Heme Ecology Evolution Behavior and Systematics 030304 developmental biology Regulation of gene expression 0303 health sciences 030306 microbiology Membrane Proteins Hypoxia (medical) Adaptation Physiological Cell biology Mitochondria Oxygen Oxidative Stress chemistry Mitochondrial Membrane Protein Mitochondrial Membranes Nuclear transport medicine.symptom Reactive Oxygen Species Oxidative stress |
| Zdroj: | Environmental microbiologyReferences. 22(7) |
| ISSN: | 1462-2920 |
| Popis: | Adaptation to low-oxygen (LO) environment in host tissues is crucial for microbial pathogens, particularly fungi, to successfully infect target hosts. However, the underlying mechanisms responsible for hypoxia tolerance in most pathogens are poorly understood. A mitochondrial protein, BbOhmm, is demonstrated to limit oxidative stress resistance and virulence in the insect fungal pathogen, Beauveria bassiana. Here, we found that BbOhmm negatively affected hypoxic adaptation in the insect haemocoel while regulating respiration-related events, heme synthesis and mitochondrial iron homeostasis. A homologue of the mammalian sterol regulatory element-binding proteins (SREBPs), BbSre1, was shown to be involved in BbOhmm-mediated LO adaptation. Inactivation of BbSre1 resulted in a significant increase in sensitivity to hypoxic and oxidative stress. Similar to ΔBbOhmm, ΔBbSre1 or the ΔBbOhmmΔBbSre1 double mutant accumulated high levels of heme and mitochondrial iron, regulating the similar pathways during hypoxic stress. BbSre1 transcriptional activity and nuclear import were repressed in ΔBbOhmm cells and affected by intracellular reactive oxygen species (ROS) and oxygen levels. These findings have led to a new model in which BbOhmm affects ROS homeostasis in combination with available oxygen to control the transcriptional activity of BbSre1, which in turn mediates LO adaptation by regulating mitochondrial iron homeostasis, heme synthesis and respiration-implicated genes. |
| Databáze: | OpenAIRE |
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