XNP-1/ATR-X acts with RB, HP1 and the NuRD complex during larval development in C. elegans

Autor: Michel Fontes, Cécile Mignon-Ravix, Carole Couillault, Anne Millet, Nathalie Pujol, Jonathan J. Ewbank, Carlos Cardoso
Přispěvatelé: Département de génétique médicale [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Rok vydání: 2005
Předmět:
Cell division
MESH: Helminth Proteins
MESH: DNA Helicases
SynMuv genes
Retinoblastoma Protein
Animals
Genetically Modified
0302 clinical medicine
MESH: Animals
Hepatocyte Nuclear Factor 1-alpha
Genes
Helminth
Genetics
0303 health sciences
Larval arrest
MESH: Hepatocyte Nuclear Factor 1
Nuclear Proteins
Embryo
Helminth Proteins
MESH: Transcription Factors
MESH: Caenorhabditis elegans Proteins
DNA-Binding Proteins
Larva
Hepatocyte Nuclear Factor 1
[SDV.IMM]Life Sciences [q-bio]/Immunology
RNA Interference
ATR-X syndrome
Mi-2 Nucleosome Remodeling and Deacetylase Complex
MESH: Mutation
Transgene
MESH: RNA Interference
Chromatin remodelling
Biology
Histone Deacetylases
MESH: Animals
Genetically Modified
MESH: Gonads
03 medical and health sciences
MESH: Caenorhabditis elegans
Animals
Humans
Functional studies
MESH: Hepatocyte Nuclear Factor 1-alpha
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Gonads
Molecular Biology
Gene
030304 developmental biology
MESH: Humans
DNA Helicases
Cell Biology
MESH: Retinoblastoma Protein
Mi-2/NuRD complex
MESH: Histone Deacetylases
Mutation
Heterochromatin protein 1
MESH: Larva
MESH: Nuclear Proteins
MESH: DNA-Binding Proteins
030217 neurology & neurosurgery
Function (biology)
Transcription Factors
MESH: Genes
Helminth
SWI/SNF complex
Developmental Biology
Zdroj: Developmental Biology
Developmental Biology, Elsevier, 2005, 278 (1), pp.49-59. ⟨10.1016/j.ydbio.2004.10.014⟩
Developmental Biology, 2005, 278 (1), pp.49-59. ⟨10.1016/j.ydbio.2004.10.014⟩
ISSN: 0012-1606
1095-564X
DOI: 10.1016/j.ydbio.2004.10.014
Popis: Mutations in the XNP/ATR-X gene cause several X-linked mental retardation syndromes in humans. The XNP/ATR-X gene encodes a DNA-helicase belonging to the SNF2 family. It has been proposed that XNP/ATR-X might be involved in chromatin remodelling. The lack of a mouse model for the ATR-X syndrome has, however, hampered functional studies of XNP/ATR-X. C. elegans possesses one homolog of the XNP/ATR-X gene, named xnp-1. By analysing a deletion mutant, we show that xnp-1 is required for the development of the embryo and the somatic gonad. Moreover, we show that abrogation of xnp-1 function in combination with inactivation of genes of the NuRD complex, as well as lin-35/Rb and hpl-2/HP1 leads to a stereotyped block of larval development with a cessation of growth but not of cell division. We also demonstrate a specific function for xnp-1 together with lin-35 or hpl-2 in the control of transgene expression, a process known to be dependent on chromatin remodelling. This study thus demonstrates that in vivo XNP-1 acts in association with RB, HP1 and the NuRD complex during development.
Databáze: OpenAIRE
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