Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing
| Autor: | Martial Saugy, Julien Boccard, Jean-Luc Veuthey, Nicolas Jan, François P. Pralong, Carine Schweizer, Serge Rudaz, Norbert Baume, Flavia Badoud, Raul Nicoli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_treatment
Urinary system Clinical Biochemistry Urine Pharmacology Analytical Chemistry Steroid Eating Metabolomics medicine Humans Ingestion Testosterone UHPLC-QTOF/MS General Pharmacology Toxicology and Pharmaceutics Doping in Sports ddc:615 Chromatography Chemistry Uhplc qtof ms Steroid Metabolism General Medicine Steroidomics Medical Laboratory Technology Doping analysis Steroids Multiblock data analysis |
| Zdroj: | Bioanalysis, Vol. 6, No 19 (2014) pp. 2523-2536 |
| ISSN: | 1757-6199 |
| Popis: | Aim: Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse. Results: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted. Conclusion: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study. |
| Databáze: | OpenAIRE |
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