Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers

Autor: Stéphane Bouchonnet, Nicolas Creusot, Jean-Marc Porcher, Sophie Bourcier, Said Kinani, Patrick Balaguer, Selim Ait-Aissa
Přispěvatelé: Institut National de l'Environnement Industriel et des Risques (INERIS), Département de Chimie de l'École polytechnique (X-DEP-CHIM), École polytechnique (X), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Laboratoire des mécanismes réactionnels (DCMR), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), This study was funded by the French Ministry of Ecology and Sustainable Development (Program 189), the 'Agence Française de Sécurité Sanitaire de l'Environnement et du Travail' (AFSSET, RD-2005-02) and by a doctoral fellowship from the ANRT and INERIS (to SK)., Aït-Aïssa, Selim
Jazyk: angličtina
Rok vydání: 2010
Předmět:
[SDE] Environmental Sciences
Geologic Sediments
Receptors
Steroid
Bisphenol A
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
02 engineering and technology
Endocrine Disruptors
010501 environmental sciences
Toxicology
SEDIMENT
01 natural sciences
chemistry.chemical_compound
Bioassay
media_common
MESH: Dioxins
Pregnane
Pregnane X Receptor
General Medicine
Contamination
Pollution
MESH: Endocrine Disruptors
[SDV.TOX] Life Sciences [q-bio]/Toxicology
MESH: Water Pollutants
Chemical
Receptors
Estrogen
Endocrine disruptor
Receptors
Androgen
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Environmental chemistry
[SDE]Environmental Sciences
MESH: Receptors
Androgen
MESH: Receptors
Estrogen
Biological Assay
MESH: Environmental Monitoring
Environmental Monitoring
media_common.quotation_subject
[SDE.MCG]Environmental Sciences/Global Changes
BIOESSAIS IN VITRO
Estrone
Dioxins
MESH: Biological Assay
MESH: Rivers
Rivers
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
14. Life underwater
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Persistent organic pollutant
MESH: Geologic Sediments
COURS D'EAU
Receptors
Aryl Hydrocarbon
chemistry
13. Climate action
MESH: Receptors
Aryl Hydrocarbon
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Water Pollutants
Chemical
MESH: Receptors
Steroid
Zdroj: Environmental Pollution
Environmental Pollution, Elsevier, 2010, 158 (1), pp.74-83. ⟨10.1016/j.envpol.2009.07.041⟩
Environmental Pollution, 2010, 158 (1), pp.74-83. ⟨10.1016/j.envpol.2009.07.041⟩
ISSN: 0269-7491
1873-6424
DOI: 10.1016/j.envpol.2009.07.041⟩
Popis: International audience; A comprehensive evaluation of organic contamination was performed in sediments sampled in two reference and three impacted small streams where endocrine disruptive (ED) effects in fish have been evidenced. The approach combined quantitative chemical analyses of more than 50 ED chemicals (EDCs) and a battery of in vitro bioassays allowing the quantification of receptor-mediated activities, namely estrogen (ER), androgen (AR), dioxin (AhR) and pregnane X (PXR) receptors. At the most impacted sites, chemical analyses showed the presence of natural estrogens, organochlorine pesticides, parabens, polycyclic aromatic hydrocarbons (16 PAHs), bisphenol A and alkylphenols, while synthetic steroids, myco-estrogens and phyto-estrogens were not detected. Determination of toxic-equivalent amounts showed that 28-96% of estrogenic activities in bioassays (0.2-6.3 ng/g 17beta-estradiol equivalents) were explained by 17beta-estradiol and estrone. PAHs were major contributors (20-60%) to the total dioxin-like activities. Interestingly, high PXR and (anti)AR activities were detected; however, the targeted analysed compounds could not explain the measured biological activities. This study highlighted the presence of multiple organic EDCs in French river sediments subjected to mixed diffuse pollution, and argues for the need to further identify AR and PXR active compounds in the aquatic environment.
Databáze: OpenAIRE
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