L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay
| Autor: | Sandra Rivera Fidel, Maria Aparecida Affonso Boller, Rosana Belchior Miranda |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Mineral trioxide aggregate
Dental Instruments medicine.medical_specialty Neutral red Cell Survival In vitro cytotoxicity Perforation (oil well) endodontic sealers Incubation period Calcium Hydroxide Root Canal Filling Materials Mice chemistry.chemical_compound L Cells medicine Animals Tooth Root Aluminum Compounds Cytotoxicity General Dentistry Cement Calcium hydroxide Chromatography Epoxy Resins Silicates Tooth Injuries Oxides Calcium Compounds Fibroblasts Resin Cements Surgery Drug Combinations chemistry cytotoxicity root perforation Root Canal Preparation |
| Zdroj: | Brazilian Dental Journal v.20 n.1 2009 Brazilian Dental Journal Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP Brazilian Dental Journal, Volume: 20, Issue: 1, Pages: 22-26, Published: 2009 |
| ISSN: | 0103-6440 |
| DOI: | 10.1590/s0103-64402009000100003 |
| Popis: | This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5% CO2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p0,05) e grau 2 (branda) para o MBPc (p |
| Databáze: | OpenAIRE |
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