Genotoxic and cytotoxic effects of 50 Hz 1 mT electromagnetic field on larval rainbow trout (Oncorhynchus mykiss), Baltic clam (Limecola balthica) and common ragworm (Hediste diversicolor)
| Autor: | Janina Baršienė, Tomas Makaras, Martyna Greszkiewicz, Eugeniusz Andrulewicz, Milda Stankevičiūtė, Magdalena Jakubowska, Dariusz P. Fey, Barbara Urban-Malinga, Gintarė Sauliutė, Janina Pažusienė, Zbigniew Otremba |
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| Rok vydání: | 2018 |
| Předmět: |
animal structures
Endpoint Determination Health Toxicology and Mutagenesis 010501 environmental sciences Aquatic Science medicine.disease_cause 01 natural sciences 03 medical and health sciences Electromagnetic Fields medicine Cytotoxic T cell Animals Cytotoxicity 030304 developmental biology 0105 earth and related environmental sciences Cell Nucleus 0303 health sciences biology Chemistry Aquatic animal Polychaeta Marine invertebrates biology.organism_classification Molecular biology Survival Analysis Bivalvia Larva Oncorhynchus mykiss Micronucleus test Hediste diversicolor Rainbow trout Genotoxicity Water Pollutants Chemical DNA Damage |
| Zdroj: | Aquatic toxicology (Amsterdam, Netherlands). 208 |
| ISSN: | 1879-1514 |
| Popis: | The aim of this research was to assess genotoxicity and cytotoxicity responses in aquatic animals exposed to 50 Hz 1 m T electromagnetic field (EMF). Rainbow trout (Oncorhynchus mykiss) at early stages of development were exposed to EMF for 40 days, whereas marine benthic invertebrates – the common ragworm Hediste diversicolor and the Baltic clam Limecola balthica – for 12 days. To define genotoxicity and cytotoxicity responses in selected animals, assays of nuclear abnormalities in peripheral blood erythrocytes of O. mykiss, coelomocytes of H. diversicolor and gill cells of L. balthica were performed. Induction of formation of micronuclei (MN), nuclear buds (NB), nuclear buds on filament cells (NBf) and cells with blebbed nuclei (BL) were assessed as genotoxicity endpoints, and 8-shaped nuclei, fragmented (Fr), apoptotic (Ap) and binucleated (BN) cells as cytotoxicity endpoints. Exposure to EMF affected all studied species but with varying degrees. The strongest responses to EMF treatment were elicited in L. balthica, in which six out of the total eight analyzed geno- and cytotoxicity endpoints were significantly elevated. Significantly induced frequencies of MN were detected in O. mykiss and H. diversicolor cells, NBf and BL only in gill cells of L. balthica, and NB in analyzed tissues of all the test species. As cytotoxicity endpoints, a significant elevation in frequencies of cells with 8-shaped nuclei was found in O. mykiss and L. balthica, while Ap and BN was observed only in L. balthica. EMF exposure did not induce any significant cytotoxic activity in H. diversicolor coelomocytes. The present study is the first to reveal the genotoxic and cytotoxic activity of 1 m T EMF in aquatic animals, and, consequently, the first one to report the adverse effect of this factor on common marine invertebrates and early life stages of fish. |
| Databáze: | OpenAIRE |
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