Recombination between North American strains of porcine reproductive and respiratory syndrome virus
| Autor: | Beverly J. Schmitt, Michael P. Murtaugh, Christopher J. Nelsen, Shishan Yuan, Kay S. Faaberg |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Swine viruses Molecular Sequence Data Differential RT-PCR Genome Article law.invention Arterivirus chemistry.chemical_compound law Virology Animals Porcine respiratory and reproductive syndrome virus Amino Acid Sequence Recombination Genetic Genetics Base Sequence biology Nucleic acid sequence Porcine reproductive and respiratory syndrome virus biology.organism_classification Infectious Diseases chemistry DNA Viral North America Recombinant DNA Viral recombination Homologous recombination Recombination DNA |
| Zdroj: | Virus Research |
| ISSN: | 0168-1702 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV), a recently discovered arterivirus swine pathogen, was shown to undergo homologous recombination. Co-infection of MA-104 cells with two culture-adapted North American PRRSV strains resulted in recombinant viral particles containing chimeric ORF 3 and ORF 4 proteins. Nucleotide sequence analysis of cloned recombinant PCR products, encompassing 1182 bases of the 15.4 kb viral genome, revealed six independent recombination events. Recombinant products persisted in culture for at least three passages, indicating continuous formation of recombinant viruses, growth of recombinant viruses in competition with parental viruses, or both. The frequency of recombination was estimated from |
| Databáze: | OpenAIRE |
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