Osteopontin is involved in migration of eosinophils in asthma
| Autor: | S. Ashino, Masaharu Nishimura, Nobuyuki Hizawa, Toshimitsu Uede, Masatsugu Kurokawa, Takashi Nishimura, S.K. Huang, Ayumu Takahashi, Shigeyuki Kon, Satoshi Konno, K. Ito |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Lung Neoplasms Immunology Antibodies Antigen-Antibody Reactions Mice Eosinophil migration stomatognathic system Cell Movement Blocking antibody Immunology and Allergy Medicine Eosinophilia Animals Humans Osteopontin Aged Mice Inbred BALB C biology medicine.diagnostic_test business.industry respiratory system Eosinophil Middle Aged Asthma respiratory tract diseases Eosinophils Ovalbumin Disease Models Animal Bronchoalveolar lavage medicine.anatomical_structure biology.protein Sputum Cytokines Female medicine.symptom Bronchial Hyperreactivity business Bronchoalveolar Lavage Fluid |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 39(8) |
| ISSN: | 1365-2222 |
| Popis: | Summary Background Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. Objective To elucidate the role of OPN in eosinophilic airway inflammation. Methods OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin α4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. Results Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. Conclusion These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma. |
| Databáze: | OpenAIRE |
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