Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing
| Autor: | Yukiko Tsuji, Wout Boerjan, John Ralph, Bert F. Sels, Riet De Rycke, Lisanne de Vries, Andreas Pallidis, Ruben Vanholme, Paula Oyarce, Yanding Li, Geert Goeminne, Sander Van den Bosch, Barbara De Meester, Fernando Campos de Assis Fonseca |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Lignocellulosic biomass Plant Science complex mixtures 01 natural sciences Ferulic acid Cell wall 03 medical and health sciences chemistry.chemical_compound DEHYDROGENASE ALKALINE-DEGRADATION SACCHARIFICATION Enzymatic hydrolysis Arabidopsis CELL-WALL Lignin Science & Technology biology IDENTIFICATION Chemistry FRACTIONATION fungi Plant Sciences food and beverages biology.organism_classification POPLAR 030104 developmental biology Biochemistry Curcumin synthase Curcumin VISUALIZATION Life Sciences & Biomedicine FERULIC ACID 010606 plant biology & botany LIGNIN |
| Popis: | Lignin is the main cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric (Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby significantly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14–24% as compared with the wild type. These results demonstrate that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency. A study introduces curcumin biosynthesis in Arabidopsis by expressing the turmeric genes DIKETIDE-CoA SYNTHASE and CURCUMIN SYNTHASE 2, and the monomers curcumin and phenylpentanoids were successfully incorporated into the lignin cell wall to enhance biomass processing. |
| Databáze: | OpenAIRE |
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