Polymorphism analysis of Chinese Theileria sergenti using allele-specific polymerase chain reaction of the major piroplasm surface protein gene
| Autor: | Zhijie Liu, Gui Quan Guan, Jian Xun Luo, Qiao Yun Ren, Hong Yin, Qing Li Niu, Ai Hong Liu, You Quan Li, Qi Bai, Mi Ling Ma, Jin Liang Gao, Neil LeBlanc, Jun Long Liu |
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| Rok vydání: | 2011 |
| Předmět: |
China
Sequence analysis Protozoan Proteins Cattle Diseases Antigens Protozoan Polymerase Chain Reaction law.invention law Phylogenetics Theileria Animals Allele Gene Ecology Evolution Behavior and Systematics Polymerase chain reaction Alleles Phylogeny Genetics Polymorphism Genetic Phylogenetic tree biology Base Sequence DNA Protozoan biology.organism_classification Virology Theileriasis Parasitology Cattle Primer (molecular biology) Sequence Alignment |
| Zdroj: | The Journal of parasitology. 97(1) |
| ISSN: | 1937-2345 |
| Popis: | Theileria sergenti is a tick-borne parasite found in many parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a marker for epidemiological and phylogenetic studies of benign Theileria species. In this study, Chinese species of T. sergenti were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the MPSP gene. Using universal or allele-specific primer sets for PCR amplification of the MPSP gene, 98 of 288 cattle blood samples, collected from 6 provinces in China, were found to be positive. Among the positive samples, only 3 allelic MPSP gene types (Chitose [C]-, Ikeda [I]-, and buffeli [B]-type) were successfully amplified. Moreover, the results revealed that the majority of the parasites sampled in this study were C- and I-type (prevalence of 84 and 69%, respectively), whereas the B-type was less common (prevalence of 36%). Co-infections with C-, I-, and B-type T. sergenti also were found. An additional known allele, Thai-type, was not detected. Phylogenetic analysis based on the MPSP gene sequences, including 3 standard stocks generated in the laboratory ( T. sergenti Wenchuan, T. sergenti Ningxian, and T. sergenti Liaoyang), revealed that the isolates of Chinese sergenti were comprised of at least 4 allelic MPSP gene types, i.e., C-, I-, B1-, and B2-type, and these parasites with 6 MPSP types 1-5 and 7 were present in China. |
| Databáze: | OpenAIRE |
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