Activity of an interleukin 1 receptor antagonist in rabbit models of uveitis
| Autor: | Richard S. Boney, James T. Rosenbaum |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Intracrine medicine.drug_class medicine.medical_treatment Sialoglycoproteins Inflammation Cell Count Injections Aqueous Humor Internal medicine Arthus Reaction Escherichia coli Medicine Animals Eye Proteins business.industry Arthus reaction Interleukin Proteins Receptor antagonist medicine.disease Uveitis Anterior Recombinant Proteins Endotoxins Ophthalmology Disease Models Animal Interleukin 1 Receptor Antagonist Protein Endocrinology Cytokine Interleukin 1 receptor antagonist Recombinant Interleukin-1 Receptor Antagonist Female Rabbits medicine.symptom business Interleukin-1 |
| Zdroj: | Archives of ophthalmology (Chicago, Ill. : 1960). 110(4) |
| ISSN: | 0003-9950 |
| Popis: | Interleukin 1 has been implicated in intraocular inflammation. The availability of a cloned, recombinant interleukin 1 receptor antagonist has enabled us to test the role of interleukin 1 in specific models of uveitis in New Zealand white rabbits. Seventy-five micrograms of interleukin 1 receptor antagonist injected intravitreally resulted in a 97% reduction in aqueous humor cells present 6 hours after intravitreal injection of 10 ng of human interleukin 1 alpha. Disruption of the blood aqueous barrier was prevented by the receptor antagonist (mean +/- SD aqueous humor protein of 0.6 +/- 0.1 g/L in rabbits treated with interleukin 1 receptor antagonist vs 32.2 +/- 9.9 g/L in controls). Lower doses of interleukin 1 produced more modest but significant inhibition. Despite the activity of interleukin 1 receptor antagonist in inhibiting interleukin 1-induced inflammation, interleukin 1 receptor antagonist did not produce significant reduction in inflammation subsequent to an active Arthus reaction or subsequent to the intravitreal injection of 125 ng of endotoxin. A potential explanation of these observations is that cytokines in addition to interleukin 1 may be present in sufficient quantities to produce intraocular inflammation or that the effects of interleukin 1 may be primarily intracellular (intracrine) and therefore resistant to the activity of exogenously administered receptor antagonist. |
| Databáze: | OpenAIRE |
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