BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin's lymphoma-derived cell line DEV
| Autor: | Klaas Kok, Tjasso Blokzijl, Reiner Siebert, Joost Kluiver, Sibrand Poppema, Inge Davelaar, Josẻ Ignacio Martin-Subero, Cigdem Atayar, Eva van den Berg, Pieter van der Vlies, Anneke Bosga, Anke van den Berg, Geert Harms, Birgit Sikkema-Raddatz |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Stem Cell Aging Leukemia and Lymphoma (SALL), Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
Male HIGH EXPRESSION BCL6 ABR breakpoint PROTEIN Biology DIAGNOSIS DISEASE Immunophenotyping Pathology and Forensic Medicine Cell Line Tumor hemic and lymphatic diseases medicine Humans Lymphocytes REED-STERNBERG CELLS In Situ Hybridization Fluorescence 17q24 deletion Genetics medicine.diagnostic_test Hodgkin's lymphoma MUTATIONS Homozygote Breakpoint REARRANGEMENTS Chromosome Mapping DEV Chromosome Breakage Middle Aged medicine.disease BCL6 Hodgkin Disease Immunohistochemistry GENE DNA-Binding Proteins Chromosome 17 (human) NLPHL PATTERN Reed–Sternberg cell Chromosome 3 Cytogenetic Analysis Proto-Oncogene Proteins c-bcl-6 GROWTH Chromosome Deletion Chromosomes Human Pair 17 Microsatellite Repeats Fluorescence in situ hybridization Comparative genomic hybridization |
| Zdroj: | Human Pathology, 37(6), 675-683. W B SAUNDERS CO-ELSEVIER INC |
| ISSN: | 0046-8177 |
| Popis: | DEV is the only cell line derived from nodular lymphocyte predominance type of Hodgkin's lymphoma (NLPHL); however, a comprehensive report about the genetic and immunophenotypic profile of this unique cell line is lacking. We analyzed DEV with respect to immunophenotype and genetic aberrations. The immunostaining revealed positivity for CD45, CD20, CD22, CD79a, IgA2, CD80, CD86, CD74, and BCL6. Cytogenetically, DEV has complex chromosome 3 translocations involving chromosomes 7, 14, and 22. A detailed analysis of the 3q27 breakpoint of the der(3)t(3;14)(p14;q32)t(3;22)(q27;q11.2) revealed a break in the BCL6 alternative breakpoint region. Using array comparative genomic hybridization, a 3-megabase homozygous deletion at 17q24.1-24.2 was identified. Fluorescence in situ hybridization indicated the presence of 2 chromosome 17 homologues, each of which carried a small interstitial deletion. Eight microsatellite markers flanking the homozygously deleted region all showed a homozygous pattern suggesting loss of one of the parental alleles. D17S1809 and D17S1816 could not be amplified using DEV DNA, in keeping with a location within the homozygously deleted segment. In conclusion, DEV has an immunophenotype that is consistent with the neoplastic cells of NLPHL cases, the lymphocytic and histiocytic cells. We demonstrated involvement of the BCL6 gene based on the presence of a breakpoint in the alternative breakpoint region and nuclear staining for BCL6 protein and identified a homozygously deleted region at 17q24. (c) 2006 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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