Comparative evaluation of microplate enzyme-linked immunosorbent assay versus plaque reduction assay for antiviral susceptibility testing of herpes simplex virus isolates
| Autor: | S. Safrin, E. Palacios, Bradley J. Leahy |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Foscarnet
viruses Acyclovir Enzyme-Linked Immunosorbent Assay Microbial Sensitivity Tests medicine.disease_cause Antiviral Agents Herpesviridae Virus Microbiology Alphaherpesvirinae medicine Humans Pharmacology (medical) Aciclovir Pharmacology chemistry.chemical_classification biology virus diseases Herpes Simplex biochemical phenomena metabolism and nutrition biology.organism_classification Virology In vitro Infectious Diseases Enzyme Herpes simplex virus chemistry medicine.drug Research Article |
| Zdroj: | Antimicrobial agents and chemotherapy. 40(4) |
| ISSN: | 0066-4804 |
| Popis: | We tested the antiviral susceptibilities of 30 clinical isolates of herpes simplex virus using the microplate in situ enzyme-linked immunosorbent assay (MISE) and the plaque reduction assay (PRA). There was concordance for 26 of 30 acyclovir results and all 30 foscarnet results. MISE and PRA results each predicted the response to acyclovir in 12 of 14 instances and the response to foscarnet in 8 instances. MISE is more rapid than PRA, has an objective endpoint, and correlates well with the clinical response to therapy. |
| Databáze: | OpenAIRE |
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