Role of Candida species from HIV infected children in enamel caries lesions: an in vitro study
| Autor: | Karol de Oliveira Martins, Maristela Barbosa Portela, Senda Charone, Rosangela Maria de Araújo Soares, Gloria Fernanda Barbosa de Araújo Castro |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Molar Time Factors HIV Infections 0302 clinical medicine Reference Values Hiv infected Candida albicans Hardness Tests Child Candida Virulence Enamel paint Corpus albicans Child Preschool visual_art visual_art.visual_art_medium Female Original Article Microscopy Polarization HIV infections Dental Plaque chemistry.chemical_element Dental Caries In Vitro Techniques Biology Calcium Microbiology 03 medical and health sciences stomatognathic system Hardness Humans Enamel caries Tooth Deciduous Dental Enamel General Dentistry Analysis of Variance Spectrophotometry Atomic Biofilm 030206 dentistry biochemical phenomena metabolism and nutrition lcsh:RK1-715 Demineralization stomatognathic diseases 030104 developmental biology chemistry lcsh:Dentistry Biofilms Dental caries |
| Zdroj: | Journal of Applied Oral Science Journal of Applied Oral Science, Vol 25, Iss 1, Pp 53-60 Journal of Applied Oral Science, Volume: 25, Issue: 1, Pages: 53-60, Published: FEB 2017 Journal of Applied Oral Science v.25 n.1 2017 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1678-7757 |
| DOI: | 10.1590/1678-77572016-0021 |
| Popis: | Objectives This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Material and Methods Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C. albicans (Group 1); mixed biofilm formed by C. albicans and C. tropicalis (Group 2); mixed biofilm formed by C. albicans and C. parapsilosis (Group 3); mixed biofilm formed by C. albicans, C. parapsilosis and C. glabrata (Group 4); biofilm formed by C. albicans ATCC (Group 5) and medium without Candida (Group 6). Enamel blocks from each group were removed on days 3, 5, 8 and 15 after biofilm formation to evaluate the TMH and images of enamel were analyzed by PLM. The quantity of Ca2+ released, from Groups 1 and 6, was determined using an Atomic Absorption Spectrophotometer. The SPSS program was used for statistical analysis and the significance level was 5%. Results TMH showed a gradual reduction in enamel hardness (p |
| Databáze: | OpenAIRE |
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