Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice
| Autor: | Hiroshi Obayashi, Akiko Amano, Toru Sasaki, Michiaki Fukui, Setsuko Handa, Goji Hasegawa, Toyotaka Mori, Yasunori Sato, Yoshitaka Kondo, Akihito Ishigami, Ryoya Takahashi, Mitsuhiro Ohta, Naoto Nakamura, Hikohito Fujinawa, Shizuo Kajiyama, Naoki Maruyama |
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| Rok vydání: | 2008 |
| Předmět: |
Senescence
medicine.medical_specialty Biophysics Ascorbic Acid medicine.disease_cause Models Biological Biochemistry Antioxidants Mice chemistry.chemical_compound Superoxides Internal medicine medicine Animals Lucigenin Molecular Biology Mice Knockout Vitamin C Superoxide Body Weight Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Brain Water Cell Biology Ascorbic acid Oxidative Stress Endocrinology chemistry Knockout mouse Gluconolactonase Carboxylic Ester Hydrolases Oxidative stress Hydrogen |
| Zdroj: | Biochemical and Biophysical Research Communications. 375:346-350 |
| ISSN: | 0006-291X |
| Popis: | Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation. |
| Databáze: | OpenAIRE |
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