Suppression of Renal α-Dicarbonyl Compounds Generated following Ureteral Obstruction by Kidney-Specific α-Dicarbonyl/l-Xylulose Reductase

Autor:	Noboru Otsuka, Noriyuki Miyata, Jun Asami, Hiroko Odani, Kenji Maeda, Aiko Ishii, Junichi Nakagawa, Takako Sudo, Kayoko Oide, Atsushi Nakamura
Rok vydání:	2008
Předmět:	medicine.medical_specialty Mice Transgenic Oxidative phosphorylation In situ hybridization Reductase Kidney urologic and male genital diseases General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound History and Philosophy of Science Glycation Internal medicine medicine Renal fibrosis Animals Humans urogenital system General Neuroscience Methylglyoxal Glyoxal Fibrosis medicine.anatomical_structure Endocrinology chemistry 3-Deoxyglucosone Kidney Diseases Sugar Alcohol Dehydrogenases Ureteral Obstruction
Zdroj:	Annals of the New York Academy of Sciences. 1126:320-324
ISSN:	1749-6632 0077-8923
DOI:	10.1196/annals.1433.003
Popis:	Renal unilateral ureteral obstruction (UUO) causes acute generation of alpha-dicarbonyl stress substances, such as glyoxal, 3-deoxyglucosone, and methylglyoxal, in the kidneys. These alpha-dicarbonyl compounds are prone to form advanced glycation end products (AGEs) via the nonenzymatic Maillard reaction. Using transgenic (Tg) mice overexpressing a kidney-specific short-chain oxidoreductase, alpha-dicarbonyl/L-xylulose reductase (DCXR), we measured generation of alpha-dicarbonyls following UUO by means of electrospray ionization/liquid chromatography/mass spectrometry in their kidney extracts. The accumulation of 3-deoxyglucosone was significantly reduced in the kidneys of the mice Tg for DCXR compared to their wild-type littermates, demonstrating 4.91 +/- 2.04 vs. 6.45 +/- 1.85 ng/mg protein (P = 0.044) for the obstructed kidneys, and 3.68 +/- 1.95 vs. 5.20 +/- 1.39 ng/mg protein (P = 0.026) for the contralateral kidneys. Despite the reduction in accumulated alpha-dicarbonyls, collagen III content in kidneys of the Tg mice and their wild-type littermates showed no difference as monitored by in situ hybridization. Collectively, DCXR may function in the removal of renal alpha-dicarbonyl compounds under oxidative circumstances, but it is not sufficient to suppress acute renal fibrosis during 7 days UUO.
Databáze:	OpenAIRE
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