Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
| Autor: | Bilge Özaydın, Taek Soon Lee, Helcio Burd, Jay D. Keasling |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Terpenes organic chemicals Phenotypic screening Bioengineering Biology Applied Microbiology and Biotechnology Carotenoids Yeast Terpenoid chemistry.chemical_compound Biochemistry chemistry Metabolic Engineering Species Specificity Yeasts lipids (amino acids peptides and proteins) Fermentation Mevalonate pathway Bisabolene Gene Carotenoid Gene Deletion Biotechnology |
| Zdroj: | Metabolic engineering. 15 |
| ISSN: | 1096-7184 |
| Popis: | Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40mg/L to 800mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2g/L of bisabolene. |
| Databáze: | OpenAIRE |
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