In Situ Characterization of the Human Host Response to Leishmania panamensis
| Autor: | Nancy G. Saravia, Gloria I. Palma |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Pathology medicine.medical_specialty Adolescent Biopsy Antibodies Protozoan Antigens Protozoan Dermatology CD8-Positive T-Lymphocytes Lymphocyte Activation Pathology and Forensic Medicine Antigen-Antibody Reactions Necrosis Immune system Dermis Cutaneous leishmaniasis medicine Animals Humans Macrophage Fluorescent Antibody Technique Indirect Leishmaniasis Skin Leishmania Immunity Cellular Granuloma biology Immunoperoxidase General Medicine Middle Aged medicine.disease biology.organism_classification Immunohistochemistry medicine.anatomical_structure Immunology biology.protein Regression Analysis Epidermis Antibody Biomarkers Granulocytes |
| Zdroj: | The American Journal of Dermatopathology. 19:585-590 |
| ISSN: | 0193-1091 |
| DOI: | 10.1097/00000372-199712000-00006 |
| Popis: | Both host and parasite determinants influence the outcome of Leishmania infections. Human host responses in cutaneous leishmaniasis of limited duration caused by a single species of the Viannia (V) subgenus were studied in skin biopsies obtained from lesions caused by Leishmania (V) panamensis in 31 male patients from the Colombian Pacific Coast. Dermal infiltrates and histopathologic changes were characterized using monoclonal antibodies and an indirect immunoperoxidase method. Dermal distribution of T-cell subpopulations and B-lymphocytes was nonrandom: CD4+ and CD8+ T cells were most frequent in the upper dermis, and B cells were most abundant in the lower dermis. Parasites, macrophages, and neutrophils were localized predominantly in the middermis. Multiple regression analyses to establish associations between lesion type (ulcer, nodule, or papule), immune response data (Montenegro skin test, indirect fluorescence antibody test titers, lymphocyte blastogenesis), and particular cell populations demonstrated statistically significant correlations between CD4+ lymphocytes and macrophages (p < 0.05). CD8+ lymphocytes were associated with plasma cells (p < 0.001), as was the presence of amastigotes (p < 0.05). These associations and the in situ divergence of CD4 and CD8 ratios suggest that prognostic indicators for disease evolution could be identified by prospective analysis of cellular relationships and response to therapy. |
| Databáze: | OpenAIRE |
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