Inhibition of adhesion of S-fimbriated Escherichia coli to epithelial cells by meconium and feces of breast-fed and formula-fed newborns: mucins are the major inhibitory component
| Autor: | R. Nobis-Bosch, R Plogmann, V. Wahn, A. Lethen, F. G. Hanisch, Horst Schroten, Jörg Hacker |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Meconium Fimbria Blotting Western Biology medicine.disease_cause Bacterial Adhesion Epithelium Microbiology chemistry.chemical_compound Feces Neuraminic acid medicine Escherichia coli Humans Glycoproteins chemistry.chemical_classification Milk Human Mucin Gastroenterology Infant Newborn Mucins Pathogenic bacteria biology.organism_classification Enterobacteriaceae N-Acetylneuraminic Acid Cheek chemistry Fimbriae Bacterial Pediatrics Perinatology and Child Health Chromatography Gel Sialic Acids Infant Food Glycoprotein |
| Zdroj: | Journal of pediatric gastroenterology and nutrition. 15(2) |
| ISSN: | 0277-2116 |
| Popis: | We investigated the ability of meconium, feces from human milk-fed (HMF) newborns, and feces from formula-fed (FF) newborns to inhibit adhesion of S-fimbriated E. coli to human buccal epithelial cells. S-fimbriae are a common property of E. coli strains causing sepsis and meningitis in neonates. Meconium had the highest content of neuraminic acid and the strongest inhibitory effect on bacterial adhesion. HMF also exerted high inhibitory activity while FF was markedly less active: To achieve inhibitory effects comparable to HMF a sixfold amount of FF was required. Glycoproteins from excretions were separated by gel chromatography. Fractions obtained were analyzed for adhesion-inhibiting activity. In all excretions analyzed, the mucin-containing fraction could be identified as the major inhibitory component. Inhibition was probably mediated by specific interaction of this fraction with S-fimbriae, as shown by binding of isolated fimbriae on Western blots after electrophoretic separation of glycoproteins. In conclusion, our data support the view that the mucin-containing fraction from meconium and human milk exerts antibacterial functions by preventing adhesin-mediated binding of pathogenic bacteria to mucosal epithelia. |
| Databáze: | OpenAIRE |
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