Riboflavin and ultraviolet A irradiation for the prevention of progressive myopia in a guinea pig model
| Autor: | Luyi Zhang, Jinjing He, Miaoqin Wu, Xiaoxia Li, Lan Zhang, Hui Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty genetic structures Ultraviolet Rays Riboflavin Guinea Pigs Administration Oral Guinea pig 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Oral administration Ophthalmology medicine Animals Irradiation Dioptre Tissue Inhibitor of Metalloproteinase-2 Photosensitizing Agents Vitamin C Chemistry Fibroblasts Hyperplasia medicine.disease eye diseases Sensory Systems Biomechanical Phenomena Surgery Sclera Axial Length Eye Disease Models Animal 030104 developmental biology medicine.anatomical_structure Myopia Degenerative 030221 ophthalmology & optometry Matrix Metalloproteinase 2 sense organs |
| Zdroj: | Experimental Eye Research. 165:1-6 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2017.08.019 |
| Popis: | In this study, we evaluated the effect of oral administration of riboflavin combined with whole-body ultraviolet A (UVA) irradiation on the biochemical and biomechanical properties of sclera in a guinea pig model to control the progression of myopia. Experimental groups were administered 0.1% riboflavin solution with or without vitamin C by gavage from 3 days before myopic modeling and during the modeling process. Guinea pigs underwent 30 min of whole-body UVA irradiation after each gavage for 2 weeks. For control groups, guinea pigs were administered vitamin C and underwent either whole-body UVA irradiation without 0.1% riboflavin solution or whole-body fluorescent lamp irradiation with or without 0.1% riboflavin solution. Resultantly, myopia models were established with an increased axial length and myopic diopter. Compared with myopic eyes in the control groups, the net increase in axial length, diopter and strain assessment decreased significantly, and the net decrease in sclera thickness, ultimate load, and stress assessment decreased significantly in experimental groups. MMP-2 expression showed a lower net increase, while TIMP-2 expression showed a lower net decrease. In addition, hyperplasia of scleral fibroblasts was more active in myopic eyes of experimental groups. Overall, our results showed that oral administration of riboflavin with whole-body UVA irradiation could increase the strength and stiffness of sclera by altering the biochemical and biomechanical properties, and decreases in axial elongation and myopic diopter are greater in the guinea pig myopic model. |
| Databáze: | OpenAIRE |
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