TNF-α detection using gold nanoparticles as a surface-enhanced Raman spectroscopy substrate
| Autor: | Jose Luis Cuellar Camacho, Lizeth García-Torres, Hugo R. Navarro-Contreras, Alejandra Ortiz-Dosal, Juan Manuel Núñez-Leyva, Elizabeth Loredo-García, Eleazar Samuel Kolosovas-Machuca, Jorge Alejandro Alegría-Torres |
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| Rok vydání: | 2020 |
| Předmět: |
Biomedical Engineering
Medicine (miscellaneous) Metal Nanoparticles Bioengineering 02 engineering and technology Development Spectrum Analysis Raman 01 natural sciences symbols.namesake Dynamic light scattering Side chain General Materials Science Aqueous solution Chemistry Tumor Necrosis Factor-alpha 010401 analytical chemistry Substrate (chemistry) Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Transmission electron microscopy Colloidal gold symbols Gold 0210 nano-technology Raman spectroscopy Nuclear chemistry |
| Zdroj: | Nanomedicine (London, England). 16(1) |
| ISSN: | 1748-6963 |
| Popis: | Background: TNF-α is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-α in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-α samples, combined with AuNPs, with decreasing concentrations of TNF-α. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-α/AuNPs (∼9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-α concentrations as low as 0.125 pg/ml. Conclusion: TNF-α signal at physiological concentrations was determined with SERS. |
| Databáze: | OpenAIRE |
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