Development of multipurpose recombinant reporter bovine leukemia virus
| Autor: | Jumpei Uchiyama, Fumiaki Sato, Masahiro Sakaguchi, Ken Onda, Satoshi Taharaguchi, Kenji Tsukamoto, Shinji Kamisuki, Yusuke Yajima, Sang Gun Roh, Hironobu Murakami |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
viruses Biology Virus Replication Antiviral Agents Virus law.invention 03 medical and health sciences Amprenavir Viral Envelope Proteins law Genes Reporter Virology medicine Leukemia Virus Bovine Animals Luciferase Luciferases 030304 developmental biology 0303 health sciences Bovine leukemia virus 030302 biochemistry & molecular biology Viral Envelope Gene biology.organism_classification Viral replication Recombinant DNA Antiviral drug medicine.drug |
| Zdroj: | Virology. 548 |
| ISSN: | 1096-0341 |
| Popis: | Bovine leukemia virus (BLV) is a global problem that results in significant economic losses to the livestock industry. We developed three virus strains by inserting the HiBiT reporter tag from NanoLuc luciferase (NLuc) into limited sites within BLV molecular clones. Initial analysis for site selection of the tag insertion revealed a permissible site immediately downstream of the viral envelope gene. Therefore, NLuc activity could be used to measure virus copy numbers in the supernatant and the levels of cell infection. Productivity and growth kinetics of the reporter virus were similar to those of the wild-type strain; therefore, the reporter virus can be used to characterize the replication of chimeric viruses as well as responses to the antiviral drug, amprenavir. Collectively, our results suggest that the BLV reporter virus with a HiBiT tag insertion is a highly versatile system for various purposes such as evaluating virus replication and antiviral drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect