Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
| Autor: | Corinne M. LaViolette, Margaret M. Briehl, Venessa Jim, Jani C. Ingram |
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| Rok vydání: | 2017 |
| Předmět: |
inorganic chemicals
0301 basic medicine microprobe Laser ablation inductively coupled plasma mass spectrometry medicine.medical_treatment lcsh:RS1-441 chemistry.chemical_element Spatial distribution mice kidney complex mixtures Article uranium lcsh:Pharmacy and materia medica lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound medicine LA-ICP-MS Inductively coupled plasma mass spectrometry Kidney Radiochemistry technology industry and agriculture Uranium Ablation 030104 developmental biology medicine.anatomical_structure lcsh:QD1-999 Uranyl nitrate chemistry Toxicity |
| Zdroj: | Journal of Applied Bioanalysis, Vol 3, Iss 3, Pp 43-48 (2017) Journal of applied bioanalysis |
| ISSN: | 2405-710X |
| DOI: | 10.17145/jab.17.007 |
| Popis: | The aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in their drinking water. Calibration standards were developed to allow for semi-quantitative measurement of uranium in the cortical and medullary regions of mice kidney by LA-ICP-MS. Scanning electron microscopy was used to image the ablation patterns on the kidney. Uranium levels were observed to increase in kidney tissue as uranyl nitrate treatment exposure levels increased. A trend towards a higher uranium concentration in the medullary versus cortical region of the kidneys was observed. These results show the usefulness of LA-ICP-MS in toxicity studies by providing a quantitative, spatial assessment of uranium deposition in a target organ. |
| Databáze: | OpenAIRE |
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