Gene and stem cell therapy in ischemic stroke
| Autor: | Koji Abe, Kentaro Deguchi, Shoko Nagotani, Toru Yamashita, Tatsushi Kamiya |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Dihydropyridines
Azelnidipine medicine.medical_treatment Biomedical Engineering Ischemia lcsh:Medicine Pharmacology Neuroprotection Models Biological Antioxidants Brain Ischemia Neurotrophic factors medicine Glial cell line-derived neurotrophic factor Animals Humans Glial Cell Line-Derived Neurotrophic Factor Stroke Transplantation biology business.industry lcsh:R Biliverdine Cell Biology Stem-cell therapy Genetic Therapy medicine.disease Neural stem cell Disease Models Animal Neuroprotective Agents nervous system biology.protein business Azetidinecarboxylic Acid medicine.drug Stem Cell Transplantation |
| Zdroj: | Cell Transplantation, Vol 18 (2009) |
| ISSN: | 1555-3892 |
| Popis: | Possible strategies for treating ischemic stroke include neuroprotection (preventing injured neurons from undergoing apoptosis in the acute phase of cerebral ischemia) and stem cell therapy (the repair of disrupted neuronal networks with newly born neurons in the chronic phase of cerebral ischemia). First, we estimated the neuroprotective effect of glial cell line-derived neurotrophic factor (GDNF) by administration of GFNF protein. GDNF protein showed a direct protective effect against ischemic brain damage. Pretreatment of animals with adenoviral vector containing GDNF gene (Ad-GDNF) 24 h before the subsequent transient middle cerebral artery occlusion (MCAO) effectively reduced infarcted volume. Secondly, we studied the neuroprotective effect of a calcium channel blocker, azelnidipine, or a by-product of heme degradation, biliverdin. Both azelnidipine and biliverdin had a neuroprotective effect in the ischemic brain through their antioxidative property. Lastly, we developed a restorative stroke therapy with a bioaffinitive scaffold, which is able to provide an appropriate platform for newly born neurons. In the future, we will combine these strategies to develop more effective therapies for treatment of strokes. |
| Databáze: | OpenAIRE |
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