Lineage-specific T-cell reconstitution following in vivo CD4+ and CD8+ lymphocyte depletion in nonhuman primates
| Autor: | Engram, J.C., Cervasi, B., Borghans, J.A.M., Klatt, N.R., Gordon, S.N., Chahroudi, A., Else, J.G., Mittler, R.S., Sodora, D.L., de Boer, R.J., Brenchley, J.M., Silvestri, G., Paiardini, M., Theoretical Biology and Bioinformatics, Sub Theoretical Biology & Bioinformatics |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
lymphocytes primates Lymphocyte T cell Immunology Simian Acquired Immunodeficiency Syndrome CD8-Positive T-Lymphocytes Lymphocyte Activation medicine.disease_cause Biochemistry Cercocebus atys Species Specificity T-Lymphocyte Subsets lymphocyte depletion medicine Animals Homeostasis antibodies Cell Lineage infections t-lymphocytes Immunobiology Interleukin-15 biology interleukin-7 Antibodies Monoclonal Cell Biology Hematology T lymphocyte Simian immunodeficiency virus Macaca mulatta Molecular biology Immunity Innate medicine.anatomical_structure Interleukin 15 biology.protein Antibody Cell Division CD8 |
| Zdroj: | Blood, 116(5), 748. American Society of Hematology |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Many features of T-cell homeostasis in primates are still unclear, thus limiting our understanding of AIDS pathogenesis, in which T-cell homeostasis is lost. Here, we performed experiments of in vivo CD4+ or CD8+ lymphocyte depletion in 2 nonhuman primate species, rhesus macaques (RMs) and sooty mangabeys (SMs). Whereas RMs develop AIDS after infection with simian immunodeficiency virus (SIV), SIV-infected SMs are typically AIDS-resistant. We found that, in both species, most CD4+ or CD8+ T cells in blood and lymph nodes were depleted after treatment with their respective antibodies. These CD4+ and CD8+ lymphocyte depletions were followed by a largely lineage-specific CD4+ and CD8+ T-cell proliferation, involving mainly memory T cells, which correlated with interleukin-7 plasma levels. Interestingly, SMs showed a faster repopulation of naive CD4+ T cells than RMs. In addition, in both species CD8+ T-cell repopulation was faster than that of CD4+ T cells, with CD8+ T cells reconstituting a normal pool within 60 days and CD4+ T cells remaining below baseline levels up to day 180 after depletion. While this study revealed subtle differences in CD4+ T-cell repopulation in an AIDS-sensitive versus an AIDS-resistant species, such differences may have particular relevance in the presence of active SIV repli cation, where CD4+ T-cell destruction is chronic. |
| Databáze: | OpenAIRE |
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