Ginsenoside compound K reduces the progression of Huntington's disease via the inhibition of oxidative stress and overactivation of the ATM/AMPK pathway
| Autor: | A-Ching Chao, Sheau-Long Lee, Wan-Tze Chen, Yu-Chieh Lee, Tz-Chuen Ju, Hsin-Min Wang, Wan-Han Hsu, Ding-I. Yang, Ting-Yu Lin, Kuo-Feng Hua |
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| Rok vydání: | 2021 |
| Předmět: |
Genetically modified mouse
Huntingtin Chemistry DNA damage AMPK medicine.disease_cause medicine.disease Biochemistry Genetics and Molecular Biology (miscellaneous) Complementary and alternative medicine Huntington's disease medicine Cancer research Phosphorylation Protein kinase A Oxidative stress Biotechnology |
| Zdroj: | Journal of ginseng research. 46(4) |
| ISSN: | 1226-8453 |
| Popis: | Background Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion of trinucleotide CAG repeat in the Huntingtin (Htt) gene. The major pathogenic pathways underlying HD involve the impairment of cellular energy homeostasis and DNA damage in the brain. The protein kinase ataxia-telangiectasia mutated (ATM) is an important regulator of the DNA damage response. ATM is involved in the phosphorylation of AMP-activated protein kinase (AMPK), suggesting that AMPK plays a critical role in response to DNA damage. Herein, we demonstrated that expression of polyQ-expanded mutant Htt (mHtt) enhanced the phosphorylation of ATM. Ginsenoside is the main and most effective component of Panax ginseng. However, the protective effect of a ginsenoside (compound K, CK) in HD remains unclear and warrants further investigation. Methods This study used the R6/2 transgenic mouse model of HD and performed behavioral tests, survival rate, histological analyses, and immunoblot assays. Results The systematic administration of CK into R6/2 mice suppressed the activation of ATM/AMPK and reduced neuronal toxicity and mHTT aggregation. Most importantly, CK increased neuronal density and lifespan and improved motor dysfunction in R6/2 mice. Conversely, CK enhanced the expression of Bcl2 protected striatal cells from the toxicity induced by the overactivation of mHtt and AMPK. Conclusions Thus, the oral administration of CK reduced the disease progression and markedly enhanced lifespan in the transgenic mouse model (R6/2) of HD. |
| Databáze: | OpenAIRE |
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