Wild blueberry proanthocyanidins shape distinct gut microbiota profile and influence glucose homeostasis and intestinal phenotypes in high-fat high-sucrose fed mice
Autor: | André Marette, Maria Carolina Rodríguez-Daza, Geneviève Pilon, Yves Desjardins, Stéphanie Dudonné, Emile Levy, Laurence Daoust, Denis Roy, Thibault V. Varin, Lemia Boutkrabt |
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Rok vydání: | 2020 |
Předmět: |
Blood Glucose
Male 0301 basic medicine Colon Blueberry Plants Administration Oral lcsh:Medicine Gut flora Carbohydrate metabolism Diet High-Fat Article Mice 03 medical and health sciences Flavonols Dietary Sucrose Glucose Intolerance Animals Humans Glucose homeostasis Proanthocyanidins Obesity Food science Intestinal Mucosa lcsh:Science 2. Zero hunger chemistry.chemical_classification 030109 nutrition & dietetics Multidisciplinary biology Plant Extracts Microbiota lcsh:R food and beverages biology.organism_classification Gastrointestinal Microbiome Gut Epithelium Disease Models Animal Glucose 030104 developmental biology Proanthocyanidin chemistry Next-generation sequencing Dysbiosis lcsh:Q Insulin Resistance Akkermansia muciniphila Vaccinium |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Blueberries are a rich source of polyphenols, widely studied for the prevention or attenuation of metabolic diseases. However, the health contribution and mechanisms of action of polyphenols depend on their type and structure. Here, we evaluated the effects of a wild blueberry polyphenolic extract (WBE) (Vaccinium angustifolium Aiton) on cardiometabolic parameters, gut microbiota composition and gut epithelium histology of high-fat high-sucrose (HFHS) diet-induced obese mice and determined which constitutive polyphenolic fractions (BPF) was responsible for the observed effects. To do so, the whole extract was separated in three fractions, F1) Anthocyanins and phenolic acids, F2) oligomeric proanthocyanidins (PACs), phenolic acids and flavonols (PACs degree of polymerization DP 4) and supplied at their respective concentration in the whole extract. After 8 weeks, WBE reduced OGTT AUC by 18.3% compared to the HFHS treated rodents and the F3 fraction contributed the most to this effect. The anthocyanin rich F1 fraction did not reproduce this response. WBE and the BPF restored the colonic mucus layer. Particularly, the polymeric PACs-rich F3 fraction increased the mucin-secreting goblet cells number. WBE caused a significant 2-fold higher proportion of Adlercreutzia equolifaciens whereas oligomeric PACs-rich F2 fraction increased by 2.5-fold the proportion of Akkermansia muciniphila. This study reveals the key role of WBE PACs in modulating the gut microbiota and restoring colonic epithelial mucus layer, providing a suitable ecological niche for mucosa-associated symbiotic bacteria, which may be crucial in triggering health effects of blueberry polyphenols. |
Databáze: | OpenAIRE |
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