Effect of Dureping injection on TIR signal pathway on Ana-1 cells
| Autor: | Ningping Wang, Ligang Gu, Pengtao Li, Yanli Zhang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cells
Biology Virus Mice Animals Pharmacology (medical) RNA Messenger General Pharmacology Toxicology and Pharmaceutics Cells Cultured Adaptor Proteins Signal Transducing TNF Receptor-Associated Factor 6 Messenger RNA Strain (chemistry) Reverse Transcriptase Polymerase Chain Reaction Macrophages NF-kappa B Transcription Factor RelA Signal transducing adaptor protein Epithelial Cells IRAK4 NFKB1 Virology Molecular biology Interleukin-1 Receptor-Associated Kinases Complementary and alternative medicine Cell culture Myeloid Differentiation Factor 88 Signal transduction Signal Transduction |
| Zdroj: | China Journal of Chinese Matera Medica. 35 |
| ISSN: | 1001-5302 |
| DOI: | 10.4268/cjcmm20100618 |
| Popis: | Objective To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway. Method Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR. Result Dureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly. Conclusion Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway. |
| Databáze: | OpenAIRE |
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