Bioimaging structural signatures of the oomycete pathogen Sclerospora graminicola in pearl millet using different microscopic techniques
Autor: | Lam-Son Phan Tran, H. S. Shetty, Hans Jørgen Lyngs Jørgensen, Sharada Mysore Suryanarayan, Aditya Rao Shimoga Janakirama, Michael Sass Hansen, Sudisha Jogaiah |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Pennisetum Cell Survival Germ tube Sclerospora graminicola lcsh:Medicine Calcofluor-white Biology 01 natural sciences Article Fluorescence imaging Microbiology 03 medical and health sciences Imaging Three-Dimensional Haustorium Fluorescence microscope lcsh:Science Plant Diseases Oomycete Microscopy Peronospora Appressorium Multidisciplinary Cell wall Sporangium fungi lcsh:R biology.organism_classification Plant Leaves 030104 developmental biology Biological Assay lcsh:Q 010606 plant biology & botany |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Shetty, H S, Suryanarayan, S M, Jogaiah, S, Janakirama, A R S, Hansen, M, Jørgensen, H J L & Tran, L S P 2019, ' Bioimaging structural signatures of the oomycete pathogen Sclerospora graminicola in pearl millet using different microscopic techniques ', Scientific Reports, vol. 9, no. 1, 15175 . https://doi.org/10.1038/s41598-019-51477-2 Scientific Reports |
ISSN: | 2045-2322 |
Popis: | In this case study, the mycelium growth of Sclerospora graminicola in the infected tissues of pearl millet and the process of sporulation and liberation of sporangia and zoospores were observed using four different microscopic techniques. The cotton blue-stained samples observed under light microscope revealed the formation of zoospores with germ tubes, appressoria and initiation of haustorium into the host cells, while the environmental scanning electron microscopy showed the rapid emergence of sporangiophores with dispersed sporangia around the stomata. For fluorescence microscopy, the infected leaf samples were stained with Fluorescent Brightener 28 and Calcofluor White, which react with β-glucans present in the mycelial walls, sporangiophores and sporangia. Calcoflour White was found to be the most suitable for studying the structural morphology of the pathogen. Therefore, samples observed by confocal laser scanning microscopy (CLSM) were pre-treated with Calcofluor White, as well as with Syto-13 that can stain the cell nuclei. Among the four microscopic techniques, CLSM is ideal for observing live host-pathogen interaction and studying the developmental processes of the pathogen in the host tissues. The use of different microscopic bioimaging techniques to study pathogenesis will enhance our understanding of the morphological features and development of the infectious propagules in the host. |
Databáze: | OpenAIRE |
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