Organ culture of postnatal rat liver: Effects of ethanol on lipid metabolism
| Autor: | George B. Reed, Kenneth D. Konrad |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
medicine.medical_specialty
Clinical Biochemistry Palmitic Acids Biology Organ culture Pathology and Forensic Medicine Palmitic acid chemistry.chemical_compound Organ Culture Techniques Internal medicine medicine Animals Pyruvates Molecular Biology Beta oxidation Phospholipids Triglycerides chemistry.chemical_classification Esterification Ethanol Triglyceride Fatty Acids Fatty acid Lipid metabolism Lipid Metabolism Lipids Culture Media Rats Blood Endocrinology Liver chemistry Lipogenesis Lactates Cholesterol Esters Oxidation-Reduction Explant culture |
| Zdroj: | Experimental and Molecular Pathology. 26:425-433 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/0014-4800(77)90044-2 |
| Popis: | Organ culture explants of 3- to 5-day-old postnatal rat liver were used to examine in vitro hepatocyte lipid metabolism as affected by incubation with 1.0% ethanol, v/v, 173 m M ) for 6 hr. Lipogenesis, as measured by [ 14 C]acetate incorporation into explant lipids, and fatty acid esterification, measured by [ 14 C]palmitate incorporation into triglycerides, were both increased by ethanol administration. Production of 14 CO 2 by the explants from [ 14 C]palmitate, [ 14 C]acetate, and [ 14 C]glucose was decreased following incubation with ethanol. Ethanol did not affect the release of labeled lipids from the explants into the media. Increases in the explant lactate/pyruvate ratio during incubation with ethanol and incorporation of [ 14 C]ethanol into fatty acids indicated that the explants were oxidizing ethanol to acetate. Explant viability, as judged by [ 3 H]leucine incorporation into cellular protein and morphologic appearance by light and electron microscopy, was unaffected by incubation with 1.0% ethanol for up to 24 hr. Despite apparently increased lipogenesis and decreased fatty acid oxidation by the explants during ethanol administration, no statistically significant ethanol-induced increases in explant triglyceride or fatty acid concentration were found unless the explants were incubated with media supplemented to 25% serum as an exogenous lipid source. These results indicate that acute hepatic explant lipid accumulation during ethanol administration is due primarily to deposition of exogenous lipids, with increased lipogenesis contributing only minimally to the increased deposition of lipids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|