Circadian rhythms in Neurospora crassa: oscillation in the level of an adenine nucleotide
Autor: | D P Delmer, S Brody |
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Rok vydání: | 1975 |
Předmět: |
Adenosine monophosphate
Light Pyruvate Kinase Microbiology Neurospora crassa chemistry.chemical_compound Adenosine Triphosphate Adenine nucleotide medicine Energy charge Molecular Biology Chromatography biology Adenine Nucleotides Computers Phosphotransferases AMP deaminase Spores Fungal biology.organism_classification Adenosine Adenosine Monophosphate Circadian Rhythm Adenosine Diphosphate Neurospora Adenosine diphosphate chemistry Biochemistry Sulfurtransferases Mutation Adenosine triphosphate Research Article medicine.drug |
Zdroj: | Journal of Bacteriology. 121:548-553 |
ISSN: | 1098-5530 0021-9193 |
Popis: | In a mutant strain (bd) of Neurospora, the biological clock is visibly expressed at the growing front of a mycelial mat by sequential periods of conidiating (spore-forming) and non-conidiating growth. The edges (8 mm) of the mycelium at different ages were sampled during a 31 h period, and the adenine nucleotide levels were enzymatically assayed. In the edge region, the total adenosine 5'-monophosphate (AMP) level showed an oscillation, with a minimum of 0.5 mumol/g (residual dry weight) and a maximum of 6.0 mumol/g. The total adenosine 5'-triphosphate level and the total adenosine 5'-diphosphate level showed no obvious oscillation. The oscillation in AMP content had many of the properties of a circadian rhythm. Its period was about 22 h long, it was phase-shifted by light, and it was damped out by continuous illumination. The oscillation in AMP level led to an oscillation in the overall cellular energy charge from 0.65 to 0.93. However, the energy charge calculation does not take into account any possible compartmentalization of AMP, and therefore must be interpretated cautiously. It is suggested that the underlying cause of the oscillation in AMP level could be a rhythmic, partial uncoupling of mitochondrial oxidative phosphorylation. |
Databáze: | OpenAIRE |
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