Abnormal Sperm in Mice Lacking the Taf7l Gene
| Autor: | David C. Page, Mary L. Goodheart, Martin Kouadio, George L. Gerton, Yong Cheng, Irwin Davidson, Peijing Jeremy Wang, Mariano G. Buffone |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2007 |
| Předmět: |
Male
Litter Size Cellular differentiation RNA polymerase II MESH: Litter Size Mice 0302 clinical medicine Cell Movement Transcription (biology) Testis MESH: Animals MESH: Cell Movement Sperm motility Oligonucleotide Array Sequence Analysis Epididymis Mice Inbred BALB C 0303 health sciences MESH: Testis MESH: Spermatozoa MESH: Transcription Factor TFIID Cell Differentiation Articles TAF7 Spermatozoa Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female MESH: Spermatogenesis Germ cell MESH: Cell Differentiation MESH: Mutation MESH: Epididymis MESH: Mice Inbred BALB C Biology MESH: Gene Expression Profiling 03 medical and health sciences medicine Animals Spermatogenesis MESH: Mice Molecular Biology 030304 developmental biology Gene Expression Profiling MESH: Fertility [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology Sperm Molecular biology MESH: Male Fertility Mutation MESH: Oligonucleotide Array Sequence Analysis Transcription Factor TFIID biology.protein MESH: Female |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2007, 27 (7), pp.2582-9. ⟨10.1128/MCB.01722-06⟩ |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.01722-06 |
| Popis: | TFIID is a general transcription factor required for transcription of most protein-coding genes by RNA polymerase II. TAF7L is an X-linked germ cell-specific paralogue of TAF7, which is a generally expressed component of TFIID. Here, we report the generation of Taf7l mutant mice by homologous recombination in embryonic stem cells by using the Cre-loxP strategy. While spermatogenesis was completed in Taf7l(-/Y) mice, the weight of Taf7l(-/Y) testis decreased and the amount of sperm in the epididymides was sharply reduced. Mutant epididymal sperm exhibited abnormal morphology, including folded tails. Sperm motility was significantly reduced, and Taf7l(-/Y) males were fertile with reduced litter size. Microarray profiling revealed that the abundance of six gene transcripts (including Fscn1) in Taf7l(-/Y) testes decreased more than twofold. In particular, FSCN1 is an F-action-bundling protein and thus may be critical for normal sperm morphology and sperm motility. Although deficiency of Taf7l may be compensated in part by Taf7, Taf7l has apparently evolved new specialized functions in the gene-selective transcription in male germ cell differentiation. Our mouse studies suggest that mutations in the human TAF7L gene might be implicated in X-linked oligozoospermia in men. |
| Databáze: | OpenAIRE |
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