Optical protein detection based on magnetic clusters rotation
| Autor: | Aurélien Daynes, Donatien Ramiandrisoa, Elodie Brient-Litzler, Jean Baudry, Jérôme Bibette, Eric Compain |
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| Přispěvatelé: | Laboratoire Colloïdes et Matériaux Divisés (LCMD), Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Bertin Technologies (Bertin Technologies), Bertin Technologies, HORIBA Medical (HORIBA ABX SAS), HORIBA Scientific [France] |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Optics and Photonics [SDV.BIO]Life Sciences [q-bio]/Biotechnology Scattering Chemistry Isotropy Analytical chemistry Bioengineering General Medicine Molecular physics Magnetic field Suspension (chemistry) Magnetic anisotropy Magnetics C-Reactive Protein Limit of Detection [CHIM.ANAL]Chemical Sciences/Analytical chemistry Particle Magnetic nanoparticles Colloids Molecular Biology [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] Biotechnology |
| Zdroj: | New Biotechnology New Biotechnology, Elsevier, 2015, 32 (5), ⟨10.1016/j.nbt.2015.03.013⟩ |
| ISSN: | 1871-6784 1876-4347 |
| DOI: | 10.1016/j.nbt.2015.03.013⟩ |
| Popis: | International audience; In this paper we present a simple method to quantify aggregates of 200 nm magnetic particles. This method relies on the optical and magnetic anisotropy of particle aggregates, whereas dispersed particles are optically isotropic. We orientate aggregates by applying short pulses of a magnetic field, and we measure optical density variation directly linked to this reorientation. By computing the scattering efficiency of doublets and singlets, we demonstrate the absolute quantification of a few % of doublets in a well dispersed suspension. More generally, these optical variations are related to the aggregation state of the sample. This method can be easily applied to an agglutination assay, where target proteins induce aggregation of colloidal particles. By observing only aligned clusters, we increase sensitivity and we reduce the background noise as compared to a classical agglutination assay: we obtain a detection limit on the C-reactive protein of less than 3 pM for a total assay time of 10 min. |
| Databáze: | OpenAIRE |
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