Probing functionalized gold colloids for single particle tracking experiments
| Autor: | André Lopez, Honoré Mazarguil, Frédéric Daumas, Claire Millot, Laurence Salomé |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Biophysics Receptors Opioid mu Nanotechnology Conjugated system Transfection Biochemistry Epitope Cell Line Colloid Molecule Animals Colloids Isoelectric Point Surface plasmon resonance Molecular Biology Immunoglobulin Fragments Microscopy Binding Sites Chemistry Cell Membrane Sodium Cell Biology Immunogold labelling Hydrogen-Ion Concentration Surface Plasmon Resonance Lipids Rats Kinetics Membrane Particle Gold |
| Zdroj: | Biochemical and biophysical research communications. 295(3) |
| ISSN: | 0006-291X |
| Popis: | Functionalized submicroscopic particles are currently used to label proteins or lipids at the surface of living cells for single particle tracking experiments. In many cases, it can be of crucial importance for the particle to be anchored to a single molecule. We have addressed this question for the labeling at the plasma membrane of NRK cells of the mu-opioid receptor bearing a T7 epitope at the N-terminus. Using biophysical methods we were able to prepare quasi-monovalent anti-T7 antibody conjugated gold colloids (40 nm diameter) leading to stable and specific binding to the receptor. The rational method, we report here, can be extended to design customized probes for the labeling of various tagged molecules. |
| Databáze: | OpenAIRE |
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