T cell mediated immune responses to Toxoplasma gondii in pregnant women with primary toxoplasmosis

Autor: Paola Taverna, Vito Pistoia, Ignazia Prigione, R. Ceccarelli, R. Frulio, Marie-France Cesbron-Delauw, Paola Bocca, Fabio Morandi, Sabrina Chiesa
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)
Rok vydání: 2005
Předmět:
Cellular immunity
T-Lymphocytes
Protozoan Proteins
Lymphocyte Activation
MESH: Pregnancy Complications
Parasitic
MESH: Recombinant Proteins
0302 clinical medicine
MESH: Pregnancy
Pregnancy
hemic and lymphatic diseases
Cytotoxic T cell
MESH: Animals
MESH: Protozoan Proteins
Cells
Cultured
0303 health sciences
MESH: Cytokines
biology
MESH: Toxoplasma
Recombinant Proteins
3. Good health
Infectious Diseases
medicine.anatomical_structure
MESH: Toxoplasmosis
Cytokines
Female
Toxoplasma
Toxoplasmosis
MESH: Cells
Cultured
MESH: Immunophenotyping
T cell
Immunology
CD4-CD8 Ratio
Antigens
Protozoan
Microbiology
Peripheral blood mononuclear cell
Immunophenotyping
03 medical and health sciences
Immune system
Antigen
medicine
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Lymphocyte Activation
030304 developmental biology
MESH: Humans
Toxoplasma gondii
biology.organism_classification
MESH: T-Lymphocytes
Pregnancy Complications
Parasitic
MESH: CD4-CD8 Ratio
MESH: Female
CD8
030215 immunology
MESH: Antigens
Protozoan
Zdroj: Microbes and Infection
Microbes and Infection, 2006, 8 (2), pp.552-60. ⟨10.1016/j.micinf.2005.08.008⟩
Microbes and Infection, Elsevier, 2006, 8 (2), pp.552-60. ⟨10.1016/j.micinf.2005.08.008⟩
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2005.08.008⟩
Popis: The aim of this study was to investigate T cell immunity to Toxoplasma gondii (Tg) in pregnant women with primary toxoplasmosis. This issue has never been addressed before in humans and available information derives from murine models. Peripheral blood mononuclear cells (PBMC) from pregnant women with primary Tg infection were stimulated with Tg tachyzoites, excretory-secretory antigens (ESA) or recombinant surface antigen-1 (rSAG-1), and tested for proliferation, immunophenotype, cytokine production and antigen specific cytotoxic activity. Pregnant women with primary toxoplasmosis displayed a significant decrease of the CD4/CD8 T cell ratio and a significant increase of circulating T cell receptor (TCR) gammadelta+ cells as compared to their uninfected counterparts. T cells from Tg infected pregnant women proliferated to Tg tachyzoites, ESA or rSAG-1. Most tachyzoite and ESA specific T cell blasts were CD4+, whereas SAG-1 specific blasts were CD4+ and CD8+. ESA and tachyzoite specific T cell blasts displayed a Th1 or Th0 cytokine profile with overexpression of IFN-gamma. This pattern was unchanged upon in vitro exposure of T cells to progesterone, tested at a concentration close to that reached in vivo at the maternal-fetal interface. Finally, tachyzoite or ESA specific T cell blasts lysed, through a granule exocytosis dependent mechanism, autologous lymphoblastoid cell lines presenting Tg antigens. In conclusion, pregnant women with primary toxoplasmosis mounted in vitro Tg-specific Th1/Th0 responses whose impact on neonatal infection warrants further investigation.
Databáze: OpenAIRE
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